FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41047495
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG20200.013.57898326
AGGGATC132600.012.3641892
CGTGGCG91250.012.3227211
GGGAGGT194550.011.6754013
AACTAAC146100.011.40806337
GGAGGTC194700.011.2967774
ACTAACC149000.011.08010638
TGTCGTT27850.010.671926528
CTAACCA158300.010.51393639
GTGGCGA106400.010.5050092
GACGCAA46900.010.46968744
CAGGGAT173100.010.4584731
ATAGGGG138500.010.39147838
GGCATAG140000.010.37667935
GGTCACT202450.010.3422037
TGGCGAC111700.010.2683023
TTAACGG59300.010.1260083
ACTCTCC210950.010.0194711
GGGATCA169200.010.0086683
GAGGTCA227550.09.8537145
Produced by FastQC (version 0.11.5)