FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44262220
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT808210.18259590232934542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC155150.029.2482194
CAGACCG159550.028.286453
ACCGCGT162100.028.0358456
GACCGCG169050.026.9763935
CCGCGTT177300.025.5688747
CGCGTTC180500.024.965898
TCAGACC192650.024.021782
GAACGAT194500.021.43431545
AATACGG197350.020.9774432
GCGTTCT215800.020.9445179
TACGGAG200100.020.80196234
ATACGGA202100.020.60723733
CAATACG209950.020.08283431
CTCAGAC262450.019.7135261
AGAACGA216900.019.27200144
AAGAACG221050.018.81724243
ACGGAGA221700.018.81584235
CCCAATA230800.018.19998229
CGGAGAG238550.017.47735236
CGTTCTC268450.017.18856810
Produced by FastQC (version 0.11.5)