FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46060697
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT672750.1460572774224411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC129200.025.9148244
ACCGCGT134150.024.9215836
CAGACCG135300.024.8795013
GACCGCG135100.024.7647115
CCGCGTT151600.022.097237
CGCGTTC159150.021.0913588
TCAGACC175950.019.6943072
GAACGAT180550.018.43245
CTCAGAC222450.018.0783371
GCGTTCT187450.018.027149
TACGGAG187500.017.80652634
ATACGGA188300.017.80279433
AATACGG189300.017.68526632
AGAACGA200650.016.65272144
CAATACG209550.016.20262531
ACGCTAC156100.015.55478924
AACGCTA156050.015.53051123
TTCGACG11300.015.529726
ACGGAGA216500.015.51488235
AAGAACG218400.015.28900543
Produced by FastQC (version 0.11.5)