FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44092439
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1234960.28008430198202466No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG631330.1431832791105069No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG582190.13203851118328927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT355500.015.9428513
GGAGGTC338400.015.7303914
GGTCACT324000.015.7277057
GTTGTCG32400.015.271564526
GAGGTCA348900.015.1989925
ACTCTCC348950.014.65744911
TCACTCT361250.014.1549559
GTCACTC361600.014.1172118
CACTCTC364100.013.893496510
ATATAGC42400.013.7018656
AGGGATC187900.013.5575342
TTTCTAT539100.013.418691
ACCCGGC69100.013.2629825
GGCATAG260900.013.09243335
TGTCGTT37950.013.03721228
TTCTATA569450.012.9938332
GCTAAGT40000.012.65507914
TCTATAG577150.012.6214123
CTGGGAG322300.012.4546931
AGCTTAG271300.012.39758645
Produced by FastQC (version 0.11.5)