FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48153731
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG692420.14379363459915492No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT484750.10066717364019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC117650.023.8123044
CAGACCG123950.022.9469263
ACCGCGT143950.019.6770116
GACCGCG143050.019.6612425
CCGCGTT162350.017.4191957
CGCGTTC163750.017.2702668
TCACGCT308650.016.2038528
TCAGACC185800.015.9864552
CTCAGAC215800.015.9569091
TTCGACG15100.015.9423336
GCGTTCT181600.015.6719299
TACGGAG169100.015.1938434
AACGCTA291550.015.08890323
AATACGG181500.015.06226432
GCCGGCT320200.015.041851
ATACGGA170850.015.0261233
ACGCTAC292000.014.99629624
TTCACGC338550.014.95218857
CTACACG101050.014.60641845
GAAAACG271000.014.56183820
Produced by FastQC (version 0.11.5)