FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43941807
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1006620.22908024697300228No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG692480.1575902420216811No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG662200.15069931011257684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACT302100.014.6300677
GTCACTC305250.014.5749348
GGGAGGT336300.014.2389953
AGGTCAC343250.013.282396
ACTCTCC340150.013.24486211
GGAGGTC352050.013.1739164
AGCTTAG246350.012.97234345
GAGGTCA357650.012.79791455
TCACTCT354400.012.7123029
GGCATAG255500.012.63286135
CACTCTC358300.012.42327110
ATAGGGG265450.012.30355538
GTTGTCG26400.011.93325126
GAGCTTA277650.011.52609344
AGGGATC180350.011.5110812
TTTCTAT504100.011.3687641
TGGGAGG303050.011.3555372
TGGCATA286200.011.34854534
TCTATAG521000.010.879113
TTCTATA529300.010.8614922
Produced by FastQC (version 0.11.5)