FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46402584
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT796200.17158527206157312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC164000.026.8233724
ACCGCGT165550.026.405236
GACCGCG169450.025.8526675
CAGACCG174200.025.4206923
TCAGACC213700.021.4064062
CCGCGTT210400.020.7656567
CGCGTTC228850.019.111188
CTCAGAC273200.018.7477971
GCGTTCT269350.016.3127579
ACGCTAC145500.015.54361124
AACGCTA145650.015.49630223
CGCTACC154200.014.54929225
AAAACGC166450.013.58656221
CGTTCTC337150.013.52610310
CCGGTAT51600.013.1709151
GAAAACG181900.012.59301720
AAACGCT183350.012.42044722
CCCGTAT36550.012.314061
TCACGCT200550.012.0818988
TTCACGC205450.011.9580027
Produced by FastQC (version 0.11.5)