FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55189759
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1363560.2470675764320696No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA1163240.21077098742177872No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG808220.14644383571234657No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG687170.12451041868111799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC267100.027.3971584
GACCGCG280000.026.1326855
CAGACCG282700.026.116163
ACCGCGT288050.025.423136
TCAGACC327400.023.107382
CTCAGAC393800.021.5393811
TTCGACG16900.018.7706056
CCGCGTT435350.016.8057487
CCCGCGT17950.015.797521
ACGCTAC320900.015.7368824
AACGCTA321850.015.71650323
CGCTACC327200.015.38573325
CGCGTTC489850.014.9451518
AAAACGC354050.014.70664221
CCGGCTT382900.014.44535352
GAAAACG358100.014.40058620
CGTGGTT345800.014.2875341
GTTTTCG354900.014.25082845
AAACGCT367250.014.20242222
GCCGGCT336850.014.1638761
Produced by FastQC (version 0.11.5)