FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42540668
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA587080.13800441497533608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG102900.016.27433238
AGCTTAG105750.015.83281145
GGCATAG108150.015.50536635
AGGGATC122650.014.413562
GTTGTCG19350.013.83905326
TGGCATA122400.013.82893534
GAGCTTA122800.013.74441744
CATAGGG124400.013.62469837
GCATAGG130400.012.89422636
GGGAGGT195050.012.5458253
TAGGGGA192650.012.01013239
CAGGGAT163200.011.9347691
GGGATCA152050.011.907643
GGAGGTC221550.011.0858024
GGATCAT162400.010.9133094
CGTAGGG57050.010.8499937
GGTCACT224700.010.7005787
GGCGTAG58700.010.54500735
GCGTAGG58300.010.54013936
TTCCCTC158100.010.3707932
Produced by FastQC (version 0.11.5)