FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42148558
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT999410.23711605981870126No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG739560.17546507759529992No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCC716080.16989430575537126No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTATAGAGGAAGAAGG630850.14967297339092833No Hit
CCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG548570.13015154634708972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC186000.026.9419214
ACCGCGT189600.026.3190886
CAGACCG194400.025.8819433
TCAGACC217550.023.9656542
GACCGCG208550.023.9079995
CCGCGTT210750.023.6668287
CGCGTTC214950.023.2253258
TTCGACG20650.021.8989666
GAACGAT241400.020.87938345
CTCAGAC279350.020.8255041
GCGTTCT244050.020.5942679
ATACGGA251750.019.8129933
TCGACGA22550.019.7542537
CGGTATA28300.019.55921620
TACGGAG253650.019.54896734
AATACGG255650.019.51113132
CAATACG262300.019.12877531
AGAACGA265400.018.98258844
ACGGAGA272150.018.30275235
CGTTCTC291100.017.8066510
Produced by FastQC (version 0.11.5)