FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42148558
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA607770.1441971039673528No Hit
CAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGG570270.13530000243424697No Hit
CTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGA511630.12138730819687829No Hit
GCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGT439590.10429538301167979No Hit
CCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCG425170.10087415090214949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG19600.013.08848926
GGGAGGT191550.012.1647853
GGCATAG116300.012.057199535
GGAGGTC185500.011.8582834
ATAGGGG124100.011.69838838
GTCAGGA172400.011.3242471
AGCTTAG126950.011.150681545
CGTGGCG89150.010.8990651
TGGCATA132600.010.79572234
TTTCTAT294450.010.7704631
AGTTAGG33650.010.627694
TTCTATA295850.010.6054592
GAGCTTA136700.010.35533344
GTGGCGA93400.010.1863912
TGGCGAC95700.010.1530993
CATAGGG142750.09.996637
CGTCAGT135900.09.9964171
CGTAGGG64350.09.89867437
GGCGACC97800.09.8660954
TAGCGTA5950.09.8354837
Produced by FastQC (version 0.11.5)