FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40701355
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT669600.1645154074108835No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA418260.10276316353595599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC136700.026.4534784
GACCGCG137100.026.2095895
CAGACCG139000.026.1128923
ACCGCGT140500.025.6365266
TCAGACC163050.022.9790212
CTCAGAC211850.019.589741
CCGCGTT193400.018.6707927
CGCGTTC218000.016.5742248
TTCGACG10600.016.3428966
ACGCTAC117650.016.02950324
AACGCTA118650.015.89250923
CGCTACC120200.015.65199825
GCGTTCT249850.014.5244329
AAAACGC133250.014.201920521
AAACGCT136750.013.9370122
GAAAACG138900.013.60652920
CCCGCGT12750.013.5917941
GTCCGCT30250.013.01993751
CGTTCTC294300.012.78939110
GTTTTCG116250.012.75377545
Produced by FastQC (version 0.11.5)