FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40701355
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT205750.014.539073
ATAGGGG120150.014.44319738
GGCATAG123850.014.06628135
GTTGTCG15900.013.72819426
GGAGGTC219200.013.3186124
GGTCACT212700.013.2180497
AGCTTAG133200.013.09302645
AGGGATC98750.012.5954262
TGGCATA140600.012.48658434
GAGCTTA141300.012.3265944
GGCGTAG90100.011.56619635
GCGTAGG90450.011.521440536
GTCACTC243400.011.5048258
CGTAGGG90850.011.49548837
TAGGGGA247750.011.33760639
CATAGGG158450.011.07992337
TGGCGTA96200.010.9029834
AGCATAG98200.010.70158145
GGGATCA126350.010.6807273
TTTCTAT275200.010.5430461
Produced by FastQC (version 0.11.5)