FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46136116
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1043360.22614820892161797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG205800.027.0075263
AGACCGC205300.026.9417724
GACCGCG218050.025.3948655
ACCGCGT231650.023.9788766
TCAGACC263000.021.9296992
CCGCGTT273650.020.2821377
CTCAGAC310150.020.1077041
CGCGTTC286100.019.368088
GCGTTCT304400.018.248059
CGTTCTC360750.015.80302710
GAACGAT361700.015.208055545
TACGGAG371600.014.48189734
AATACGG376900.014.44664332
AGAACGA383900.014.34619144
ATACGGA379050.014.33408833
CAATACG394650.014.099931
AACGCTA102600.013.79499223
ACGCTAC104300.013.61521224
AAGAACG405350.013.5703843
CCCAATA414700.013.45219529
Produced by FastQC (version 0.11.5)