FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48025558
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1441980.30025262798612357No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG546200.11373110958960643No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA503650.10487124376566327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC269350.028.4883614
CAGACCG280550.027.5515823
GACCGCG309100.024.8602525
ACCGCGT317400.024.2928776
CTCAGAC362950.023.4957241
TCAGACC353900.022.470672
TTCGACG17150.020.3347726
CCGCGTT387800.019.893627
CGCGTTC415650.018.5654538
GCGTTCT418500.018.3798879
CGTTCTC478200.016.50410
TCGACGA23350.015.4165527
CGCGTAA42600.015.004441
GTCGCTA35200.014.51050527
GTTCTCT583700.013.706017511
CGACGTC37600.013.52479923
CTACACG80450.013.34100445
ACGCTAC238350.013.32998824
AACGCTA238600.013.21228323
GAACGAT568550.013.09160845
Produced by FastQC (version 0.11.5)