FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40420944
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1425340.3526241247606686No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT589660.14587981913534726No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT574960.14224309061163934No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC496690.12287936669663133No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG424580.10503960521060568No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGA404780.1001411545460195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG19950.026.7304426
AGACCGC139000.023.9425374
GACCGCG141200.023.4570715
CAGACCG144550.023.4142273
ACCGCGT142300.023.3548076
CCGGCTT430200.021.4416222
TCGACGA26400.020.9646347
GCCGGCT408700.020.7464471
CGGTATA31800.020.51817720
AACGCTA422400.020.50154323
TCAGACC168650.020.441962
ACGCTAC421900.020.28203624
CCGCGTT163850.020.1849177
TTCACGC446500.019.9036037
AAAACGC436400.019.89564121
CGCTACC423850.019.83894525
GAAAACG442050.019.77877220
AAACGCT447500.019.41212322
GTTCGAC26550.019.407595
TCACGCT458500.019.3287058
Produced by FastQC (version 0.11.5)