FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40532555
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1766530.4358299149905551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG349350.025.2357123
AGACCGC349150.025.21154
GACCGCG348400.025.2056265
ACCGCGT350200.025.0739356
CCGCGTT371900.023.6047297
GAACGAT364000.023.6008545
TCAGACC382000.023.3497752
CGCGTTC376300.023.3347058
CTCAGAC431800.022.0354061
GCGTTCT419450.021.025389
AATACGG410800.020.96552332
ATACGGA414750.020.74351133
AGAACGA417500.020.6302444
TACGGAG417350.020.5975834
CAATACG426050.020.3006331
CCCAATA444650.019.52339229
AAGAACG441800.019.52099643
ACGGAGA441050.019.48055835
CGTTCTC464550.019.1730410
CCCCAAT464500.018.79212828
Produced by FastQC (version 0.11.5)