FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40532555
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG565690.13956435758860994No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA562320.13873292714954683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT283050.014.8198053
GTTGTCG28900.014.79442126
GGAGGTC290900.014.2652514
GTCACTC289600.014.1042498
GGTCACT289550.013.9667757
GAGGTCA325000.013.0038945
AGCTTAG223600.012.77250345
ACTCTCC320150.012.62492711
ATAGGGG230850.012.43146238
GGCATAG230500.012.29391835
TCACTCT338250.012.1621469
TCTATAG343650.012.0493683
TGTCGTT37350.011.92992428
TTTCTAT362450.011.9270091
CACTCTC344600.011.6965310
TTCTATA365800.011.6087412
GAGCTTA247700.011.54795644
ATATAGC46250.011.4770516
TGGCATA251300.011.38383434
CTATAGT370450.011.1412334
Produced by FastQC (version 0.11.5)