FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27891794
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1551570.5562818942374234No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC773650.2773754890058345No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG409570.14684247273588782No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA389230.13955000528112319No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG373570.1339354506920566No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG360940.12940723712501245No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGC334720.12000662273642204No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC328250.11768694405243348No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGC318350.11413751299037989No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT316830.11359254983741812No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGA301890.10823613568922816No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGG292260.1047835072925033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG148550.025.58962244
TCGGCGC152950.024.94202245
AGACCGC322300.024.526684
CAGACCG325750.024.2956753
ACCGCGT332400.023.8197356
GACCGCG334750.023.5870955
TCAGACC347350.022.8951532
CCGCGTT348100.022.7583437
CGCGTTC355050.022.3255218
CTCAGAC390700.021.6053811
GCGTTCT374800.021.1789789
CGCGTAA41700.020.1941451
TTCGACG11050.019.7453446
AATACGG402200.019.37002632
ATACGGA402650.019.33035333
CGTTCTC413900.019.282110
CTACACG106700.019.26801945
TACGGAG404550.019.17631734
TTCGCGC30650.019.007487
ACGGAGA421600.018.59083435
Produced by FastQC (version 0.11.5)