FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27891794
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA890980.3194416250170211No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG751940.26959183765662403No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG352740.12646730432613978No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG345260.12378551196814375No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC332610.11925012783329748No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA323040.11581901113997901No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT317310.1137646434646692No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC308290.11053071738590928No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA285460.10234551423977961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC86000.020.1421265
TCACCCG89200.019.5717753
ACGGACA93850.018.08306718
CGGACAC96050.017.75772313
ACACGGA96350.017.54236216
CGGACAG96200.017.4775519
CGAAGCG54950.017.4042551
ATAGCTC93300.017.19602239
CCGGACA100200.017.04209912
CACCCGG107700.016.480864
GACACGG103550.016.42930415
CACGGAC106250.015.99315917
TAGCTCT101950.015.69092740
CGACGAC60750.015.4833231
CGACCCA58450.015.3955084
GGACACG110450.015.32047514
TTGATAG114200.015.093060536
AGCTTAG121150.015.05776445
GCCCGGA116750.015.00734210
ACGACCC61200.014.8880213
Produced by FastQC (version 0.11.5)