FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005297987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005297987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31718906
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT816420.25739223162362534No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA793890.25028921237069146No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG607230.19144102889298895No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG420910.13270003700632046No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG361710.1140360893909771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC160150.025.9783974
CAGACCG163450.025.5354523
GACCGCG177950.023.3794215
TCAGACC189900.022.6541712
ACCGCGT188900.022.0588726
AAAACGC112850.020.67570721
CTCAGAC237550.019.645841
AACGCTA127000.019.59439523
ACGCTAC128650.019.46551124
GAAAACG120400.019.41646620
AAACGCT121000.019.37594222
CGCTACC136300.018.29044725
GTTTTCG141050.017.25893845
GTTCCTA78100.016.85280815
TTCGGCG67050.016.8447644
TCGGCGC71050.016.30808845
TAACGCT43250.015.71090923
CCTACTC87700.014.90543518
AATAACG47050.014.58556921
ATAACGC46450.014.48324722
Produced by FastQC (version 0.11.5)