FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26117495
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGG421510.16138990358761438No Hit
CCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTC316730.12127120154517117No Hit
GCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGT277780.10635782643013811No Hit
CGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCAC266320.10196996304584341No Hit
CCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAGCAGGT264830.10139946422886269No Hit
GGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCG261640.10017806072136705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA950.005373098414.21333734
ATAGGGG64200.010.93783238
TATCGCG1450.006812205510.86615344
AGCTTAG64000.010.65671645
GGCATAG66100.010.58869335
AGCGCTC35750.010.5703759
CGTGGCG82100.010.4773531
CGTCAGT76550.010.0897471
ATATAGC20600.09.9383736
TATAGCT21850.09.8828637
GTTGTCG16650.09.861999526
GAGCTTA71800.09.843533544
GTCAGGA99100.09.7025431
TGGCATA74850.09.56100934
TGGCGAC90800.09.5207113
CAGACGT55600.09.3555341
GGCGACC92500.09.24826054
GTGGCGA92500.09.2240652
CGCTCTC43700.09.05917111
CGACCCG93350.09.03770356
Produced by FastQC (version 0.11.5)