FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43691379
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2681130.6136519517957993No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1332820.3050533149800559No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1291400.2955731838997346No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC833060.19066919357248943No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA546140.12499948788524161No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG460640.10543040996714706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG130350.017.82455645
CCACTCA270800.017.6061211
AGACCGC886950.017.318924
CAGACCG897800.017.2627143
TTCGACG19450.017.1202586
GAACGAT856350.016.8758745
GACCGCG924550.016.6771185
TCAGACC950800.016.3476072
ACCGCGT947750.016.283036
CTCAGAC976300.016.2528921
CCCGCGT23350.015.9068241
CACTCAG490500.015.7183871
CCGCGTT1032700.014.9457657
CGCGTTC1044500.014.6498448
GCGTTCT1070250.014.3373159
AGAACGA1047600.014.067903544
CGTTCTC1134750.013.4984410
ACTCAGA588300.012.9808782
TCGACGA28050.012.6733787
GTTCTCT1229500.012.64668211
Produced by FastQC (version 0.11.5)