FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44039615
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT9320842.1164671852830685No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA3039820.6902467244547892No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG2535710.5757793295876905No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1512110.3433522295778471No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1121200.25458896495802696No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG839720.19067378313820407No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGG479560.1088928683867922No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA459620.104365126716026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT1705450.027.17793345
CAGACCG2073850.023.0977923
AGACCGC2080750.023.0333274
TCAGACC2103150.022.9257642
CTCAGAC2136800.022.864811
GACCGCG2099700.022.8348585
ACCGCGT2111850.022.7322226
AGAACGA2085950.022.51868444
CCGCGTT2179950.022.0306637
CGCGTTC2196150.021.9096818
CTACACG203450.021.6626345
GCGTTCT2238050.021.5668459
CGTTCTC2302750.021.10938510
CCACTCA373400.020.5991551
GTTCTCT2397950.020.27018711
CCTCTCA2404400.019.97321719
AAGAACG2363000.019.89088443
AATACGG2386300.019.8773632
ACTCCCC2418750.019.80027825
ATACGGA2396800.019.78173833
Produced by FastQC (version 0.11.5)