FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42045436
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1380280.32828295561021176No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA452980.10773583130402073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC352600.021.2086054
GACCGCG353400.021.1409195
CAGACCG359350.020.956363
ACCGCGT360400.020.7802376
GAACGAT380100.018.98425945
TCAGACC401650.018.9734042
CCGCGTT399650.018.7225277
CGCGTTC410250.018.27178
CTCAGAC452250.017.847631
GCGTTCT455850.016.5574179
CTACACG65950.016.4567245
AGAACGA461800.015.90277844
AATACGG481550.015.27400432
ATACGGA487500.015.17067833
TACGGAG483700.015.14565934
TTCGACG18800.015.0776696
CGTTCTC508800.015.05096910
AAGAACG494300.014.85721343
CAATACG500850.014.75314731
ACGGAGA510050.014.40320335
Produced by FastQC (version 0.11.5)