FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28451947
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG482260.169499823685177No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG341370.11998124416582107No Hit
GAAAAAAATTCCAAAAGAGAACCACACACTACCATATCCAAACAACTTTTG318560.11196421812538875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG10250.021.5080876
AGACCGC52950.020.0958794
TCGTTAC30850.019.90733744
AACGCTA180350.018.90162523
ACGCTAC180450.018.79162624
GACCGCG55450.018.6213975
CAGACCG57800.018.6060353
CGCTACC183950.018.37372425
CGTTACC34150.017.9836445
CCGCGTT109650.017.917041
AAAACGC193850.017.66585521
GTTTTCG179400.017.60558145
AAACGCT198950.017.2016322
CGTGGTT193550.017.03882441
TCGACGA12850.016.9811947
GAAAACG198750.016.94637720
ACCGCGT62250.016.6233316
TCGTGGT200000.016.46685640
CGCGTTC120800.016.203342
TCGACAC38750.016.0276235
Produced by FastQC (version 0.11.5)