FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33897306
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT588000.17346511253726182No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG430240.1269245408469924No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG394470.11637207983430896No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA367110.10830064194482004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC133150.022.8811234
CAGACCG138250.022.2166353
GACCGCG139600.021.8386045
ACCGCGT148150.020.638346
TTCGACG16100.019.5640666
TCAGACC170700.018.69192
CTACACG68450.017.48636845
CTCAGAC210700.016.7091141
CCGCGTT193550.015.7973137
CGGTATA23650.015.79304720
TCGACGA20600.015.5087947
CCGTACG77200.014.8645834
CGCGTTC206450.014.8320168
CGTACGC79400.014.4800965
AACGCTA199300.013.62682523
ACGCTAC199250.013.61899324
TCACGCT206450.013.6005558
TCCGTAC89550.013.19181253
CGCTACC206250.013.12385125
GTACGCC88500.013.1178716
Produced by FastQC (version 0.11.5)