FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45028019
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1654990.3675467046418364No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA1631720.36237881129080984No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1022440.22706750656741082No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG859500.1908811489130801No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC451940.10036861714924655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC200100.025.2278774
CAGACCG204150.024.8730833
TCAGACC248350.021.0261542
GACCGCG245250.020.6935525
CCCGCGT25400.019.4912471
TTCGGCG113850.019.32441944
CTCAGAC300450.018.6948971
ACCGCGT275200.018.4251466
TCGGCGC123300.018.04398345
CCGCGTT476950.017.056351
CTACACG36600.016.90249645
CCGTACG143800.015.6126514
CGTACGC145350.015.492595
CGCGTTC532000.015.2582162
GTCCGTT28750.015.0284451
TACGCCA156950.014.6055657
CGCCGCG154100.014.46201828
GTACGCC157650.014.3837466
TCCGTAC160150.014.27296453
AGTTGCA576800.014.14565344
Produced by FastQC (version 0.11.5)