FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70998519
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG171800.011.97852945
ATAGGGG181750.011.78746938
GGCATAG184050.011.27379135
GAGCTTA202650.010.51178644
CATAGGG202100.010.31106137
TGGCATA216550.09.76887434
GCATAGG213100.09.74755336
GGGAGGT322750.09.2405373
GCGTTCG30550.08.91284415
ATTTCTA461500.08.8132172
GGAGGTC320300.08.7280514
CGCGTTC33550.08.51786914
AGGGATC187150.08.4383012
CGTAGGG84550.08.40938340
GCGTAGG84000.08.35757739
ACTCTCC334450.08.17957211
TCTATAG495550.08.1727265
TAATCGG57800.08.1324534
GTCACTC338000.08.0380198
GGCGTAG87550.08.019670538
Produced by FastQC (version 0.11.5)