FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44861698
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG744990.16606370984887822No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA485350.10818805832984743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC59600.018.723724
CAGACCG66200.017.096433
CCGCGTT175650.015.8095541
AACGCTA156150.015.66266223
ACGCTAC159550.015.371924
CCCGTAT21600.015.314971
CTACACG40300.015.12841345
CGCTACC162800.014.98318825
GTCCGTT27800.014.9754371
CGCGTTC191000.014.3720762
GTTTTCG165600.014.318892545
AAAACGC173150.014.2546121
CGTGGTT172850.013.91325541
GCGTTCT203650.013.7224813
GAAAACG182050.013.64263620
AAACGCT181350.013.59766322
TCGGCGC77850.013.553246545
TTCGGCG80700.012.90740744
CGGCAGT302400.012.885076540
CGCGTGT10050.012.7632521
Produced by FastQC (version 0.11.5)