FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40819512
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTT48250.011.61063735
TTACGGT50300.011.13760434
GTAACGA93300.010.31981718
TGTTACG54750.010.31459332
ACGGTTG55150.010.23943136
CGTGGCG88450.09.9014721
CTAGAAT110300.09.8382881
ATAGGGG87950.09.79714138
CTTAATC65050.09.7945132
CGGTTGG58650.09.70267737
ATCGGTT102550.09.6319886
GTGTCGA100850.09.56998927
GGTAACG100600.09.52628517
TAATCGG99700.09.507074
GTCACTC125450.09.4168928
GGTCACT126800.09.31635957
CACCGAA222850.09.2999145
CGTAACG28800.09.29731442
TCTATAG232250.09.2480743
GGCATAG91900.09.180572535
Produced by FastQC (version 0.11.5)