FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34647815
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1371330.3957911920275492No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG934700.26977170133239287No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG757680.21868045647322926No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT583040.1682761236170304No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG569250.16429607465867618No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA384020.11083527200777307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG186050.016.53010245
GTCACTC288250.014.7510918
ATAGGGG210700.014.65838838
GGGAGGT303150.014.6503023
GGCATAG212700.014.45839835
GGTCACT297500.014.26328857
GAGCTTA220150.014.16206644
AGGTCAC325400.013.49618156
TCACTCT326300.013.39734659
GGAGGTC328600.013.3230224
TGGCATA233250.013.20378434
GAGGTCA340150.013.1599285
ACTCTCC324200.013.15211711
CACCCGG100550.012.45461
CATAGGG256200.012.2131337
TGGGAGG271550.012.1135912
TTTCTAT638950.011.9853051
CACTCTC363200.011.96200210
GTTGTCG21850.011.94242326
GCATAGG266700.011.65722936
Produced by FastQC (version 0.11.5)