FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24382223
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3804691.560436060321489No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC409870.16810198151333453No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA402200.16495624701652511No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA323880.13283448354975672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAC566600.034.6372381
TCAGACC550750.034.5395972
CAGACCG548750.034.4442863
AGACCGC558550.033.8208474
GACCGCG579400.032.6115535
ACCGCGT591500.031.9634596
CCGCGTT639150.029.5805157
CGCGTTC656450.028.722118
GCGTTCT660100.028.5530689
GAACGAT681100.027.02646645
CGTTCTC703300.026.96220410
AGAACGA701200.026.26121344
GTTCTCT728350.026.16692511
AATACGG712250.025.97834432
ATACGGA712900.025.94887733
TACGGAG715050.025.8048334
CCCAATA724200.025.63987529
CAATACG723950.025.58335931
AAGAACG727500.025.2929743
CGCGTAA21850.025.2358511
Produced by FastQC (version 0.11.5)