FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40209104
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA806930.2006834074193745No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC650920.16188373657866137No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT541950.13478290886561411No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC447230.11122605467657275No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG403840.10043496617084528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATC135850.015.0714872
GTGGCGA127500.014.346792
CGTGGCG133950.013.840761
TGGCGAC136150.013.7162153
GGGATCA161350.013.0242213
GTTGTCG25100.012.81969526
GGCGACC144850.012.799164
GCGTTCG47750.012.58074415
CAGGGAT185400.012.4391181
CGACCCG157150.012.1838386
TTTCTAT371150.012.1000331
GCGACCC159350.012.0438665
CGTCGAT23350.011.9482212
CCGCGTT52100.011.91899913
GGGAGGT227700.011.9167583
CGTAGGG81800.011.8823337
GGCGTAG82050.011.84674435
GCGTAGG81200.011.83170336
ATAGGGG119750.011.81798838
CGCGTTC53550.011.59626314
Produced by FastQC (version 0.11.5)