FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4695764
Sequences flagged as poor quality0
Sequence length116-123
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT1549903.3006343589669327No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC1375572.929384866871504No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1268642.70166899358656No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG1171802.4954405715449073No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT945092.0126437359288074No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT723441.5406225696180644No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC696451.483145234726447No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG610361.2998097860113924No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC577301.2294059071111751No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG567661.2088767663792304No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC540391.1508031493916646No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT497591.059657171868092No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG486341.035699409084443No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT480311.0228580482324068No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC453990.966807531213238No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT448380.954860593505125No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT447630.9532634093195484No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG407310.8673987875029494No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC307060.6539085013642082No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC264200.5626347491057898No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG259090.5517526008547278No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG253460.5397630715683327No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT250130.5326715737843725No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT246290.5244939907542202No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC239830.5107369109691203No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT237570.5059240626232493No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT231700.49342343439746966No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC227770.48505418926504823No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG226930.48326534297720247No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG216290.4606066233311555No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT196310.41805763662739437No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC180540.3844741771520034No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG162190.3453964040782288No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT160490.34177611992425516No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT156290.33283188848502604No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG156150.3325337474370518No Hit
GGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCATGGGTACCAGTC149470.3183081602908494No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG145330.30949170358646644No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT144680.30810747729230004No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC144580.30789451940088985No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA143040.3046149678731725No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA142860.3042316436686341No Hit
GAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCCTGTCTCCTCAT140400.2989928795399428No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG139750.2976086532457764No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG137670.29317912910444394No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA137070.2919013817559826No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG136900.29153935334058523No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC133930.28521450396570186No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC131440.2799118524695875No Hit
CAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG121200.2581049643891814No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC114800.2444756593389276No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT112930.24049334676955655No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC111990.23849154259030053No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT111500.2374480489223905No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA109690.23359351108786555No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG107090.22805660591119994No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT106360.2265020133039054No Hit
GACAGTGACACTGATCTGATAAAGCCCCCATCCTGGCCTGACCCTGCCAT102640.21857997974344534No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA101920.21704668292529183No Hit
GCTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGC101020.2151300619025999No Hit
GAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCATGG100840.2147467376980615No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC99670.21225513036856195No Hit
GCTATTTCCCCAGGCAGGGCTGGGAGAGATGAGATCCTGGCCTGGACCTG99040.2109134956526776No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT98180.20908205778654973No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT96300.2050784494280377No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT95590.20356644839902516No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT94740.20175630632203834No Hit
GCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCTG94260.20073410844326928No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC94030.20024430529302578No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT94030.20024430529302578No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG92180.19630458430193679No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT87620.18659370445363097No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC87040.18535854868345172No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC84130.17916147404341445No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC83130.1770318951293123No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT81200.17292180782509514No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC80410.17123944048295445No Hit
GGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGTCTTGATTATCTCC80160.17070704575442888No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG79090.16842839631633957No Hit
GAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG78590.1673636068592885No Hit
GGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC78480.16712935317873726No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG77280.16457385848181466No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG77280.16457385848181466No Hit
GAGAGAAGGAACTGTGTCACCACAGAAACTTCTGCCTTCACCCATCCCTT77050.16408405533157117No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTG76520.16295537850709704No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG75740.16129430695409736No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC75670.1611452364301102No Hit
GCTTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC73860.1572906985955853No Hit
GACAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGT73480.1564814586082265No Hit
TTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTC72600.1546074291638166No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC72270.15390466812216286No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC72010.1533509776044963No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT70630.15041215870303534No Hit
GGATCTGATAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC69670.14836776294549725No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC69570.14815480505408704No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT69020.14698353665133085No Hit
GAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCT68480.1458335640377157No Hit
AGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT68400.14566319772458752No Hit
GGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGAC68220.14527987352004915No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC67750.14427897143042112No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG67590.1439382388041648No Hit
AGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCAT67320.1433632524973572No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC66330.14125496937239607No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT65730.13997722202393476No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC65610.1397216725542425No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC65420.1393170525605631No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC64800.13799671363381977No Hit
ACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG64700.13778375574240953No Hit
GCTCTCACCACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGC64590.13754950206185831No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC64020.13633564208082008No Hit
GGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG63690.13563288103916638No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT63690.13563288103916638No Hit
GATTCCAGCCTCTGACAAACTCTGCACATTAGGCCAGGAGAAGCCCCCGA63110.1343977252689871No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG60100.12798769273753963No Hit
GCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCCA59950.1276682559004243No Hit
GAGAGACTCCAGGATCATCCACTGAGCACTGGACATAAGGAAGGCTGCAT59930.12762566432214226No Hit
GATCCTGGTCTGACACTGTCATGGGTACCAGTCTCCTATGCTGGGTGGTC59710.1271571569610398No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA59690.12711456538275773No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT59660.12705067801533468No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA59140.12594329698000153No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC58690.12498498646865558No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG58500.12458036647497617No Hit
GGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTGT57630.12272763281970728No Hit
GCTTCCCCAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCC57230.12187580125406644No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG57210.12183320967578438No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT56790.12093878653186149No Hit
GATCCAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCC56230.11974622233996428No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC55890.11902216550916955No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG54420.11589168450543937No Hit
GGACTCTGCTCTCTGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGT54090.11518892346378566No Hit
GTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCC53900.11478430347010625No Hit
GAGATGCAATGCAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCC53320.113549147699927No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT52560.11193066772520936No Hit
CCTTGCCTGGTCTGCCTCACTCTGCCATGGGCTGCAGGCTCCTCTGCTGT52530.1118667803577863No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC52450.11169641404465813No Hit
GAGACCTTGCCTGTGGGGCCATGGGAGCTCAAAATGCCCCTCCTTTCCTC51610.1099075677568123No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC51540.10975849723282516No Hit
GGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATG51160.10894925724546634No Hit
GCTTTTCACCAATGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCT50960.10852334146264592No Hit
GGAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG50100.10669190359651805No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA50090.10667060780737703No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG50080.106649312018236No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG50040.10656412886167192No Hit
GGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCATGGGTA49400.10520119835664656No Hit
AGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT48520.10332716891223664No Hit
ACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCATG48270.10279477418371111No Hit
GCTTTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGC48040.10230497103346761No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC47440.10102722368500631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTCG302.928573E-9116.8290941
ATCTACG1050.0111.2610558
GAATGCG43250.0108.995471
GAGGTAG3500.0108.484151
GGATCTG85900.0106.832661
GCACTAC5050.0106.418591
GCCCGAC6600.0106.208261
GCTTCAA54800.0104.9969561
GACTGTA5700.0104.5312961
GAGAGCT57400.0104.311691
GACTACG8300.0104.160871
GTCTTTC12500.0103.276921
GAGAACT22900.0103.054481
GAGAGAC16650.0101.0413741
TATTAAC1450.0100.710447
GACAGAC184350.0100.637161
GGCTGTT30700.0100.465411
GCTTTTC78550.0100.245461
ATTAACG1400.0100.134968
AGTGGTA221250.099.929662
Produced by FastQC (version 0.11.5)