FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8020844
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC6556438.174239518933419No Hit
TGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATG1846902.3026255092356864No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC1229951.5334421165652892No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC1187881.4809912772271845No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG1030901.285276212827478No Hit
GGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATG1013891.2640689683030863No Hit
CGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCACCAACC793120.988823620057939No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT713660.8897567388170123No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC411470.5130008762170166No Hit
CAGTCTCCCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC385400.48049806229868075No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC370950.46248250184145206No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA369640.46084925726020853No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT307300.38312676321843436No Hit
GTTCTCTGCAGAGAGGCCTGAGTGATCCATCTCCACTCTGACGATCCAGC298420.37205560911046265No Hit
TCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCC290720.36245562187719893No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT277510.34598603339000233No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC275890.34396629581625077No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA259350.3233450245385648No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC255670.31875697869201797No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC242580.3024370003954696No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT241930.3016266118627915No Hit
CCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATCCTG234300.2921138972407393No Hit
GTGCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGC225140.2806936526879216No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG214230.26709159285481676No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT213880.26665522979875933No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT212280.26466042725678246No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA204590.25507290753940604No Hit
GTTCTCTGCAGAGAGGACTGAGGGATCCATCTCCACTCTGACGATCCAGC200660.25017317379567533No Hit
CGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGT195830.24415136362208267No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA191470.2387155266951957No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT182500.22753216494423778No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC182490.22751969742835043No Hit
GCGGTTCTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCC182450.227469827364801No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG171340.21361841721394909No Hit
CTCAAAGGAGCAGCTGCTCTGGTGGTCTCTCCCAGGCTCTGGGGGCGGAC169390.21118725161591473No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC163840.20426778029843246No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC161430.20126310896957977No Hit
AGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCACCAACC161120.20087661597707174No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA160510.20011609750794304No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA159910.19936804655470175No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG153090.19086520071952526No Hit
CTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT152720.19040390263169313No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC145650.1815893688993328No Hit
GCGCTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTAC143760.1792330083966226No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC141570.17650262241729175No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG134060.16713951798588778No Hit
GTTCTCTGCAGAGAGGCCTGAGGGAGCCATCTCCACTCTGACGATCCAGC123990.15458472948732077No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG121100.15098161739587504No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA119170.14857538682961544No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG118480.1477151282333879No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC115230.14366318556999735No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG110920.13828968622254717No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA109900.1370179996020369No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC105580.1316320327386993No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG105550.13159463019103726No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC102850.12822840090145127No Hit
TCGCTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCC101750.12685697415384217No Hit
CCCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGC98570.12289230410166312No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC97420.12145853977461724No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG96970.12089750155968623No Hit
CTTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT96540.12036139837652995No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA95550.11912711430368177No Hit
CGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATG92160.11490062641786823No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT90570.11291829139177872No Hit
AAATTCTACTAAGGAACAGGAGAGGACCTGGCAGGTGGACTTGGGGAGGC88280.1100632302535743No Hit
GTTCTCTGCAGAGAGGCCGGAGGGATCCATCTCCACTCTGACGATCCAGC88060.1097889449040525No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG87310.10885388121250084No Hit
GTTCTCTGCAGAGAGGCCTGAGGGATCCAGCTCCACTCTGACGATCCAGC87070.10855466083120431No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT86970.10842998567233075No Hit
GCGCTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCC86210.10748245446489171No Hit
GCCCAGTCTCCCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCC84490.10533804173226659No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA83370.10394167995288277No Hit
CAATCGCTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACA82760.10318116148375409No Hit
TGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGCAGGTAG82730.10314375893609201No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT81560.10168505957727143No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA80810.10074999588571977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC1377250.0144.28748145
TGTTGTC944050.0143.15225145
CGTGCTA269850.0142.30109145
TGTTGCC221700.0141.97923145
CGTGCCC286500.0141.47038145
CGTACTG230150.0141.3652145
AGTTGTC278600.0139.88747145
TTGACCC182550.0138.03798145
GGGTTGC192400.0137.690759
CGTTGTC153450.0136.95628145
TGTTGTA152300.0136.18166145
ATCGATT600850.0136.066073
TGATCGA319400.0136.029071
CGTACCG45250.0135.05469145
AGAGCGA119200.0134.901188
CCCCAAA112050.0134.777919
AGTGCTC385850.0134.6729145
GGTGCTC274450.0134.02565145
GATCGAT626950.0132.402112
CGTGCGC47750.0131.62743145
Produced by FastQC (version 0.11.5)