FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5786962
Sequences flagged as poor quality0
Sequence length108-123
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC2485444.294896009339615No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG1741623.009558383137819No Hit
GAGTCTGCCCACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCTG1398532.416691175784462No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT742771.2835232026752552No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC679151.1735864171909198No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA604841.0451770721839888No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT579931.0021320340448063No Hit
GATCCAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCC527040.9107369289793159No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT485910.8396633674110872No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG473210.8177174828519697No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC438080.7570120557211193No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG407780.7046529768123586No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC338020.5841061337537726No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT320160.5532436535785098No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG315120.5445344206511119No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT299530.5175945513379905No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG272930.4716291553322797No Hit
GGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTCT268920.4646997854832985No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC256260.4428230218204301No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT237530.4104571621517473No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT218800.37809130248306455No Hit
GAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTC211370.36525209600477765No Hit
GACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTCTA205510.3551258847042714No Hit
AGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGCT205460.3550394835839599No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG203100.3509613507052578No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT182220.31488024286318106No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA178100.3077607905495146No Hit
AGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTCT165040.2851928179241543No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA164350.2840004824638558No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC159620.2758269364823892No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT157100.27147232001869026No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT150620.26027473482632163No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT148690.25693965158229826No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC145330.2511334962973664No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG144050.24892162761739234No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT140440.24268346673090302No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT135970.23495920657505615No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT130400.22533412177235657No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT128660.2223273627855168No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT126350.21833563102712614No Hit
GAGACCTTGCCTGTGGGGCCATGGGAGCTCAAAATGCCCCTCCTTTCCTC121620.21016208504565956No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT117760.20349191855761278No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG117410.2028871107154324No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT117100.2023514237695012No Hit
GATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCTGGGTCCTGCC116270.2009171651723305No Hit
ACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAA110680.19125751992150633No Hit
GACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTCTATG108890.1881643598143551No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG105280.1819261989278658No Hit
GACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGA104470.1805265007788197No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT102250.1766902910369897No Hit
GGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTC101900.1760854831948093No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC97720.16886234953676904No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG97210.16798105810959188No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC95430.16490517822650297No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC90710.15674891246909864No Hit
GGAGTCTGCCCACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCT89900.15534921432005255No Hit
GGAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTT89870.15529737364786567No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT86170.1489036907448157No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC85610.14793599819732703No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC85500.14774591573264176No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG84110.14534396458798243No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA83750.14472187652173973No Hit
GCTTTTCACCAATGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCT78830.13622000628308947No Hit
GACAAGATCCTGCCCTGGAGCTGAAATGGGCACCAGGCTCTTCTTCTATG77310.13359341222562027No Hit
GGAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCT76330.13189995026751514No Hit
GGAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTG76160.1316061864584561No Hit
GCTCATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGC75890.13113962040877405No Hit
GCAGTCTGCCCACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCT75860.13108777973658717No Hit
GACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGCTCTTCTTCTGTG75510.13048297189440677No Hit
GCTTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC73850.12761445470006544No Hit
GGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTCTGG73450.12692324573757352No Hit
CAACATCTGGGTCATAACGCTATGTATTGGTACAAGCAAAGTGCTAAGAA71990.12440033302447812No Hit
AAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGCTCTTCTTCT71480.12351904159730097No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC71340.12327711846042881No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC71310.12322527778824191No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG69290.11973467252765785No Hit
GACACATCCCTCCAGCTAGGCAGGACAGGTAGAGAGTCCAGTGTCCTGGA68000.11750552362362152No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG67620.11684887510925422No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG66250.11448148441271949No Hit
ACACAAGATCCTGCCCTGGAGCTGAAATGGGCACGAGGCTCTTCTTCTAT65910.11389395679460139No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT65200.11266706088617828No Hit
GGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGCT64700.1118030496830634No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC64490.11144016497775518No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT61900.10696458694562017No Hit
GACAGCAGGGCTGGGAGACACAAGATCCTGCCCTGGAGCTGAAATGGGCA61530.10632521865531518No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC60780.10502920185064288No Hit
CGAGGCTCTTCTTCTATGTGGCCCTTTGTCTGCTGTGGGCAGGACACAGG60220.10406150930315422No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC59920.1035431025812853No Hit
GACACCAGCTCCTCTGGGGTTTGCCACACTCATGATGCACTGTGTAGCAA59750.10324933877222625No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC59590.1029728551872295No Hit
CAGATGGGACAGGAAGTGATCTTGCGCTGTGTCCCCATCTCTAATCACTT58010.10024257978538653No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC58010.10024257978538653No Hit
GAGAGCCCAGCACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTCAG57910.10006977754476357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)