FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5786962
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCC1871353.2337347298980017No Hit
TGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG1615162.7910326696460075No Hit
ATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT1542132.6648351933190506No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC1272282.1985283469979584No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT1018631.7602154636577878No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC828081.4309407941507133No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG762961.3184119750570333No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT700711.2108425802692329No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA665891.1506728400843138No Hit
GGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG570580.9859750245465583No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC501420.8664649949317103No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA435080.7518279885024302No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG429250.7417536178741108No Hit
CTTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGT417450.7213629534806No Hit
CTACGTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGT290580.5021287508022344No Hit
GGATGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCC289250.4998304810019488No Hit
GTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGT262510.45362316185936596No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC255650.44176892815263No Hit
CGATGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCC249010.4302948593752646No Hit
AGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG241570.41743837267291545No Hit
TGATGGATACAGTGTCTCTCGACAGGCACAGGCTAACTTCTCCCTGTCCC228480.3948185593753683No Hit
TCGATTCTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGC213530.36898462440223384No Hit
TCCTGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGA212160.36661723370569915No Hit
AAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTGCTCCCTCCCAAA210160.3631611888932397No Hit
CCAATGCTCCAAAAACTCATCCTGTACCTTGGAGATCCAGTCCACGGAGT193470.3343204949332655No Hit
ACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC176340.3047194711145503No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG154320.26666841772937167No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT145160.2508397324883073No Hit
TGATCGATTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATG142150.24563838504555588No Hit
CGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG138250.2388990976612599No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTCCACACCCTGC135250.23371503044257075No Hit
CCGATTCTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGC133340.23041450764667193No Hit
GCTGCCCAGTGATCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCA128280.22167071427114954No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT127920.22104862620490684No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC117510.20305991295605533No Hit
CTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTGCCATCC117510.20305991295605533No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA117400.20286983049137008No Hit
CTCAGATGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCA113050.19535293302427076No Hit
CAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGAGCC112130.1937631524105394No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC111720.19305466322398523No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG111610.19286458075929996No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC104350.18031913809007213No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC99510.17195550964392026No Hit
TCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC96860.16737625026741146No Hit
TAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGTCCG95940.1657864696536801No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA92910.16055056176280405No Hit
GATGGTGACCCCAAAACAATGAGGGCCTAGAATGACATAGTTGTGCTTCA92730.1602395177296827No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA87880.15185860905946852No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA87390.15101187808041594No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC86950.15025154822167486No Hit
CCAATTCTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCC85920.14847168514325826No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT83760.14473915674580204No Hit
CCCTGACTGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC82600.14273465075457553No Hit
CCCTGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGA81140.14021173804148016No Hit
CCCTGAATGCCCCAACAGCTCTCACTTCTTCCTTCACCTACACACCCTGC79900.13806899025775526No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC79820.13793074846525688No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC79640.13761970443213553No Hit
GCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCTTGTTGC79450.13729138017495188No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT76670.13248747788563325No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC76030.1313815435456462No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC76010.13134698309752163No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT73980.1278390976128753No Hit
GGATGGATACAGTGTCTCTCGACAGGCACAGGCTAACTTCTCCCTGTCCC72220.12479777817791098No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG68700.11871513930798233No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA65060.11242513774930611No Hit
CGATGGATACAGTGTCTCTCGACAGGCACAGGCTAACTTCTCCCTGTCCC64510.11147472542587976No Hit
TAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC64430.11133648363338138No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG64190.11092175825588625No Hit
TAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGCGTCTG63900.11042063175807962No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA60560.10464903692127232No Hit
TTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG59840.10340486078878693No Hit
TTTAGCCCAATGCTCCAAAAACTCATCCTGTACCTTGGAGATCCAGTCCA59010.10197060219161627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)