FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCA212.5Illumina RNA PCR Primer (96% over 30bp)
CTATCCCATCACCCGCCCAAACCTAACATTCTCCACTCTCCCTGTGAGCA16.25No Hit
CATTCCCATGAGACGCGCTAACCTAACATTCTCTACTCTGACTGTGACCT16.25No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG16.25No Hit
GTTTCCCATCAGCCGCCCATACCTAACATTCTCTACTATGACTGTGAGCA16.25No Hit
CAGCGTCTCTCGGGAGAATAACTAATCCTTACCTCTCACTGTGACATCGG16.25No Hit
CTTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGTGGTATCA16.25Illumina RNA PCR Primer (96% over 30bp)
GTTTCCCTTCAGCCGCCCAACCCTATCATTCTCAACTCTGACTGGGAGCA16.25No Hit
CATTTGCGTCAGCCCTGTCGGATCCGTCTCTACTGTGATTATGCAGCGCA16.25No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA16.25No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA16.25No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTCTGACATCGG16.25No Hit
TTAATGATACGGCGACCACCGAGATCTACACGAGTCAGCAGGTGGTATCA16.25Illumina RNA PCR Primer (96% over 30bp)
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGCCCAGTGCCC16.25No Hit
GATTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA16.25No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)