FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8028748
Sequences flagged as poor quality0
Sequence length111-124
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT4677545.8259893074237725No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT4245795.28823423029344No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT1952822.4322845853425714No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT1736332.1626410493890207No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT1101631.3721068340916915No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC1087111.354021822580557No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT952671.1865735479554222No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC906571.1291548819317783No Hit
GAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCT903051.1247706367169577No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC875021.0898585931455314No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT869951.0835437854071395No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG846081.0538131225441376No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT705910.8792279942028322No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT514390.6406851977419145No Hit
AGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGT506530.6308953774610935No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC436880.5441446163212496No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC408030.5082112428986437No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG387530.4826779966191491No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC383030.4770731376797478No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG329050.4098397408911078No Hit
GCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTG317910.3959646012055678No Hit
GATTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTC316560.39428314352374744No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG313900.3909700491284569No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC313250.39016045839276564No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG309380.3853402797048805No Hit
ATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACTG305860.38095603449006No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG299150.3725985670493083No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC298120.37131567711428975No Hit
GAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTG289480.3605543479506394No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC264540.3294909741842688No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC254300.31673680628660905No Hit
GGAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGG249730.3110447606525949No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC243220.3029363980535944No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG234520.29210033743741864No Hit
GAGAGCCTGAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCT231960.2889117954630037No Hit
CTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTT216600.26978054361651405No Hit
GAAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTC204460.2546598797222182No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCC196660.24494479089392268No Hit
GCTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTT194580.24235410053971054No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG192100.2392652005019961No Hit
GACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTC191670.23872962509223108No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG187760.23385962543599575No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC186730.23257673550097724No Hit
GGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGT185870.23150558468144722No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC184260.2295002907053503No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC179900.22406980515517488No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG175430.2185023119420363No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA171770.2139436933379899No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA171210.21324619978108664No Hit
AAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCCT164380.20473926943528428No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT162050.2018371980288832No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG159700.198910216138307No Hit
GAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG159700.198910216138307No Hit
AGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTG158920.19793870725547744No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT150110.18696563897633853No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG143840.17915620218743944No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT143380.17858326105141176No Hit
GGATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCAC142320.17726300539013057No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT139270.17346415655342529No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG135310.16853188068675215No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG134350.16733617744634655No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG131880.16425973265071964No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC130610.16267791690559974No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA123240.15349840348706922No Hit
GAGTCTGCACCTTTCATTCACAGCAGTAACACTGTTCTCCTTAACTCTGA122170.15216569258370047No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT120120.149612367955751No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG119610.14897715060928554No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC119240.14851630665204588No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT115540.14390786707964928No Hit
GAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGG113750.14167837874597633No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC111650.13906277790758909No Hit
GGTCACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTG107520.13391876292542748No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG107200.1335201951786256No Hit
GGCTCCTGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTG105660.13160208789714162No Hit
TCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT104830.13056830280387427No Hit
GACAGACCATCAACCCACTGCCTGGTCCTGGGAGAAGACCTATTCTTTCT101350.12623387855740395No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA99880.12440295797053288No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT97930.12197418576345902No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT96820.12059165389174004No Hit
GATTCCTGCCATGGGTCCTGGGCTTCTCCACTGGATGGCCCTTTGTCTCC96790.12055428816547735No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC96170.11978206315604874No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC95170.11853653894729291No Hit
GATACACACAGTGACACTTCCATGAGCACAAGGGATGTCCTTCACTCTCT93170.11604549052978125No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT91480.11394055461698388No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC90950.11328042678634327No Hit
GGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCCCTGTGTCTCC87790.10934457028667482No Hit
GAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCG87320.10875917390855959No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG86550.1078001202678176No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA84990.10585710250215849No Hit
GACACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC84810.10563290814458244No Hit
ATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCC80370.10010278065770653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)