FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8028748
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA5496176.8456127904375625No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA4880926.079304021000534No Hit
TAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC3850764.796214802108622No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC1852132.3068727527629465No Hit
AAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC1480001.843375828958637No Hit
CAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC915621.1404268760210186No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA838581.0444716909784688No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC659990.8220335225367642No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG624400.777705315947144No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT581820.7246708951383204No Hit
GCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAGGCCTGGGGG575410.7166870849601955No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT564930.7036339912524343No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG562680.7008315617827338No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT516000.6426904917180113No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG501320.6244061963334757No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT462010.5754446396872838No Hit
CGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC442620.551293925279508No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG439130.5469470457909502No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA414300.5160206796875428No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA411210.5121720098824872No Hit
CCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGAGCCCTG357760.4455987409244878No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT335950.41843385793152305No Hit
GGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC299490.37302204528028526No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA298080.37126585614593954No Hit
CAGCGATTCTCATCTCAATGCCCCAAGAACGCACCCTGCAGCCTGGCAAT272230.3390690553496012No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG261880.32617787978897833No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC242030.30145422424517493No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG238940.29760555444011944No Hit
CTCTGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGCGCA226660.2823105171565978No Hit
CAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCT213860.2663678072845231No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG209900.26143553141785No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT207670.25865801243232445No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA201760.25129696435857746No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA195830.24391100580065536No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA195530.2435373485380286No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC194680.24247865296058613No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC193130.24054809043701458No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC182130.22684732414070038No Hit
TAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGGGGGACT176760.22015885913968156No Hit
CTCTAGAGAGAAGAAGGAGCGCTGCTCCCTGATTCTGGAGTCCGCCAGCA170240.21203804129859352No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT164320.20466453798275897No Hit
TGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAA164150.20445279886727044No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG162060.20184965327097076No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC161920.20167527988174494No Hit
TCACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC152980.19054029345546777No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA150720.1877254087436796No Hit
TAACTTCCACTCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC149910.18671653413458736No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC147050.18315433489754568No Hit
CTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCA145110.18073801793255936No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC136670.1702257936106601No Hit
TCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTC136200.16964039723254484No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC132080.1645088374924708No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC129750.1616067660860697No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC125680.15653748255643346No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG123970.154407636159461No Hit
AAGCGATTCTCATCTCAATGCCCCAAGAACGCACCCTGCAGCCTGGCAAT111660.13907523314967662No Hit
TGTCACCCACCCTCCACTCCCCTCAAAGGAGCAGCTGCTCTGGTGGTCTC110200.1372567678048931No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC110190.13724431256280556No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC103800.12928541286885575No Hit
CTCTCGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA102140.1272178426823211No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG97650.12162543898500738No Hit
AGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC97150.12100267688062946No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT93170.11604549052978125No Hit
TCGATTCTTAGCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGC92150.11477505583685028No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG91040.11339252396513129No Hit
TGAGGTGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC90180.11232137314560128No Hit
CTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT89410.1113623195048593No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG83270.10371480086309845No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC83160.10357779320013531No Hit
CAACTTCCACTCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC82020.10215789560215366No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC81570.10159740970821353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)