FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6828028
Sequences flagged as poor quality0
Sequence length113-124
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC880821.29000642645285No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT783841.1479742028005744No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT778371.1399631050136292No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG765551.1211875522478818No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT458280.6711747520660432No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA438420.6420887553478105No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG386740.5664007236057028No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG377070.5522385086880136No Hit
ATATCTGTCAACGTGGAACCTCCCTGACGATCCAGTGTCAAGTCGATAGC338270.495413902813521No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG325350.4764918948779941No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT300410.43996597553495675No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC295060.4321306239517471No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA280640.4110117884695259No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC278920.40849275954931646No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC262650.38466450342617226No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT223210.3269025844650901No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG209830.3073068827485769No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG202700.29686462914329No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT199410.2920462540575405No Hit
ATGATGTTCTGGTACCGTCAGCAACCTGGACAGAGCCTGACACTGATCGC196700.2880773189565128No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT195740.2866713493266284No Hit
GCAGGTCTCTCAAATGCCGGCGTCATGCAGAACCCAAGACACCTGGTCAG194270.28451845833086803No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC190210.27857237843781546No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC179030.2621986904564539No Hit
ATGAAAATATGTTCTGGTATCGACAAGACCCAGGTCTGGGGCTACGGCTG170780.25011613894963525No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG162090.23738918469578627No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG157300.23037398206334245No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT149960.2196241726015183No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC148590.21761773677553753No Hit
CATGCTGATGGCAACTTCCAATGAGGGCTCCAAGGCCACATACGAGCAAG147110.21545020026279915No Hit
AGTCGATAGCCAAGTCACCATGATGTTCTGGTACCGTCAGCAACCTGGAC143830.2106464706940276No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG142000.20796634108706055No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG141720.20755626661167764No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA141500.20723406523816246No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG138500.20284041014477389No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC135580.19856391918720895No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT135110.19787557988924476No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC132780.1944631744333796No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC131360.192383511022509No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT130040.190450302781418No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT124340.18210235810397965No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG121680.17820665058784177No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC121170.1774597292219657No Hit
AGTCACCATGATGTTCTGGTACCGTCAGCAACCTGGACAGAGCCTGACAC118530.17359331273978373No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT114590.1678229790504667No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG110980.1625359474214224No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT109980.1610713957236262No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT109830.16085171296895678No Hit
TCAACTAGAAAAATCAAGGCTGCTCAGTGATCGGTTCTCTGCAGAGAGGC107550.15751253509798144No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG106360.15576971857760397No Hit
AGTGTCAAGTCGATAGCCAAGTCACCATGATGTTCTGGTACCGTCAGCAA101560.14873987042818218No Hit
AGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAG100750.14755358355296727No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC99590.14585470358352368No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG98880.1448148718780884No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG96500.14132923883733342No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG95860.14039192575074386No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA95850.14037728023376586No Hit
AGTGACTTGTTCTCAGAATATGAACCATGAGTATATGTCCTGGTATCGAC94960.13907382922272726No Hit
CAACATCTGGGTCATAACGCTATGTATTGGTACAAGCAAAGTGCTAAGAA94860.13892737405294764No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC94620.13857588164547655No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC91970.13469481964631663No Hit
GGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCT91710.1343140362048896No Hit
ACAGAATGTGACCCTGAGTTGTGAACAGAATTTGAACCACGATGCCATGT91680.13427009965395573No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT91010.13328885001643226No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC89350.13085769419809057No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT89220.13066730247737707No Hit
TGATGTTCTGGTACCGTCAGCAACCTGGACAGAGCCTGACACTGATCGCA89000.1303451011038619No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC88670.12986179904358916No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT86860.12721096047057803No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT85580.1253363342973989No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC84230.12335918950537403No Hit
AGGATATGGACCATGAAAATATGTTCTGGTATCGACAAGACCCAGGTCTG83780.12270014124136575No Hit
GGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGAC83740.12264155917345387No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA82970.12151385436615081No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT81110.11878978820824988No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC78360.11476227103931033No Hit
GACAGTGACACTGATCTGATAAAGCCCCCATCCTGGCCTGACCCTGCCAT78300.11467439793744255No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG78060.11432290552997146No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC77330.11325378279058024No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG76080.111423093168335No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC75790.11099837317597408No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG75670.11082262697223855No Hit
GATCCAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCC73750.10801068771246983No Hit
GGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGG72140.10565275947901796No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC71810.10516945741874521No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT71410.10458363673962673No Hit
CAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG71260.10436395398495729No Hit
ACAGAATGTAACTTTCAGGTGTGATCCAATTTCTGAACACAACCGCCTTT70840.1037488422718829No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT70250.10288475677018313No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC69860.10231358160804262No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT69670.10203531678546134No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC69660.10202067126848337No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC69630.10197673471754949No Hit
GCTTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC69370.10159595127612249No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT68330.10007281751041443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCAA45700.098.8441851
GAGAACT53900.098.756551
GGATCTG46550.096.028471
GCTTTTC125300.093.741761
GATTCCT42000.091.167031
GAATGCG10100.090.846731
CGGTTCC600.088.226161
GCACTAC6350.087.994591
AGTCATT25850.087.371634
GGACGTG7050.085.931861
GAGAGCT44600.084.665461
GATCTCA20950.084.225451
GACTGGT8150.083.7156141
CCTCATA8300.083.324036118
GTCTTTC20450.083.120981
GACTACT11450.081.162931
GCTATTT9800.081.0240251
ATGCTCA69900.080.2720952
CATTGAG15500.079.686397
ATTGAGG11600.079.097588
Produced by FastQC (version 0.11.5)