FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8965095
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC1146021.2783132805620019No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG1036021.1556151942617452No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG1004721.1207020115235813No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT983341.0968539652954041No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT957991.0685776335889356No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC844530.9420201347559619No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA816250.910475572205314No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA761800.849740019486687No Hit
TCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA714650.7971471579498043No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA703300.7844869463179142No Hit
ATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC551230.6148624191935501No Hit
TGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTG547510.610712993002305No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT526040.5867645574307914No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC517630.5773837310145625No Hit
CTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCA516000.5755655684630224No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA474780.5295872492148717No Hit
CATTGACAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGA455700.5083047084275181No Hit
CCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACA426370.4755889368712769No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA416590.46467996156203584No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG405650.4524770791609013No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC397850.4437766694050649No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG365650.4078595932335352No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC364110.40614182002533156No Hit
CTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCA353960.39482013297126245No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC340630.37995135578596767No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA324870.36237206633058544No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG322680.35992925897606215No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA304460.3396059941361469No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA304320.3394498329354011No Hit
CTTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGATCCAGC283530.3162598946246526No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT274780.3064998195780413No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC249620.278435420929728No Hit
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA242840.2708727570650395No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA237810.26526210820967316No Hit
GCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCATT233720.26069997027360003No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC230330.2569186383412557No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT227420.25367271624003984No Hit
CCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCAC222760.24847477912950167No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA219620.24497230648420346No Hit
CAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGG219410.2447380646830848No Hit
TCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC217690.24281951278820804No Hit
CAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGG214780.23957359068699216No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC214340.23908279834179114No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT195930.21854760044372087No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG195140.21766640509665544No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC194590.2170529146651541No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG187120.2087205991682185No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC186600.20814057185116275No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA186190.20768324262040727No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT180460.20129178776131207No Hit
ATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA175440.1956922932774276No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG171270.1910409203694997No Hit
CAGTGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGG170950.1906839804820808No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC167990.1873822865234557No Hit
CCCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGC166150.18532988217079685No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA158790.1771202647601615No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA156380.1744320612330377No Hit
CCGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGA155200.1731158453981804No Hit
GCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGGAGC149800.16709248479798597No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC146430.16333346160860537No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA146170.1630434479500775No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG143170.15969713650552503No Hit
CAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGAGCC140250.15644006003282732No Hit
CTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACGCCT136990.15280373492974697No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG133780.14922318168407586No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA133230.14860969125257456No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT132170.14742732787549936No Hit
CCCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCCTGG127230.14191706836346965No Hit
GTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACAT127200.14188360524902413No Hit
CAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCCATC124400.1387603812341085No Hit
TGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGCGCACAC121580.13561484847622918No Hit
TAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCACCA120710.134644418157309No Hit
GGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC119880.13371860532431615No Hit
GCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC116790.13027190453642712No Hit
GTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGC115840.12921223924565217No Hit
CTCTGAGTCAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCC110940.12374659721954982No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG110780.12356812727584035No Hit
ATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT110330.12306618055915748No Hit
CTACAACGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGT106850.11918445928347664No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG105440.11761169290453698No Hit
TGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTG104920.11703166558748121No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG103230.11514657680705001No Hit
ATTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG102960.11484540877704029No Hit
CTTCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATT102950.11483425440555846No Hit
CGGGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA102590.11443269703221215No Hit
CGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC99630.11113100307358706No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA96850.10803008780163512No Hit
CTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCCGCT96010.10709312059716043No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC95380.10639039519380442No Hit
TGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG94070.10492917252968319No Hit
CTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGGAGT94040.10489570941523765No Hit
TGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGCGCACAC93340.10411490341150874No Hit
TGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGTCGGCTG92940.10366872855223509No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC91780.10237482146034148No Hit
TCGCTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCC91120.10163863294253993No Hit
CTTCTCAGCTCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG90210.10062358513769235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)