FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16355593
Sequences flagged as poor quality0
Sequence length115-124
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT6382123.902102479561579No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT5952953.6397029444300797No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG2763551.6896666479778506No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT2741161.675977141275159No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT2407621.4720469016317537No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG2024611.2378701279739597No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT1932901.1817975661292133No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT1910511.1681080594265214No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG1528180.9343470456864511No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1428920.8736583259316859No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC1428700.8735238153700694No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT1306590.7988643395565053No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA1239840.7580526123387883No Hit
GGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAG1100860.6730786220958176No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC1051560.6429360280608597No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC1029980.6297417647895739No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG1026810.6278035898790095No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG1003650.6136432962106602No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG973770.5953743162965721No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC941080.5753872696636557No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT927000.5667785937202032No Hit
GAGAATGACTTCCTTGAGAGTCCTGTTCCCCTTTCATCAATGCACAGATA923390.5645713976864061No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT908980.555760955900529No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG872870.5336828814461206No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC868830.5312127784055277No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT793010.48485554757935095No Hit
GACAGATACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATGAGCATCAG729100.4457802294297737No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG724740.4431144746631932No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG718040.43901801665032875No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC696120.4256158734201811No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA691920.42304794451659444No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC669780.40951129072483033No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC646160.39506974770037384No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT635980.3888455771673947No Hit
GAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTC631600.38616759416794No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATGAGC610480.37325458025276126No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT602150.36816152126064766No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC594330.3633802822068267No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC592700.36238368122757764No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT559290.3419564182111893No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC543690.3324183965692959No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG538450.32921459955624965No Hit
GAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGT529450.3237118947628496No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA524580.32073432005797653No Hit
GAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCT520630.3183192440653176No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC518880.3172492736888231No Hit
GGAGAGTCCTGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC502250.3070814980539073No Hit
GATCCTTCCCTGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCC500010.3057119359719944No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT496510.3035719952190055No Hit
GAGAGTCCTGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCG491420.300459909952516No Hit
GGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCT490390.29983015595949347No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG487360.29797757867904884No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC479320.29306182906361145No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC470600.28773031953045053No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG452340.27656594291628556No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG449790.2750068432248223No Hit
GCCTGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTG445930.27264679428009736No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC443020.270867586396898No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG439390.26864816213022663No Hit
GACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATGAGCATCAGCCTCCT436390.2668139271990933No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT435060.2660007497129575No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACC421960.2579912571803419No Hit
GGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATGAGCATC409760.25053203512706634No Hit
GACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCT402550.24612375717590917No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC397980.24332960596414938No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG394240.2410429264166698No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT391000.2390619526910458No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATG381140.23303343388405423No Hit
GAGACCCCAAAATTCCGCATCCTGAAGATAGGACAGAGCATGACACTGCA371090.2268887468647575No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA369530.22593494470056819No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGGCCTGACCCTGCCAT364750.22301239704362905No Hit
GGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGGCCCTCTGTCTCC360870.22064011986602994No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG358060.2189220531472017No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT356870.2181944732911855No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC352230.2153575232643659No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG343250.2098670467038401No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC337030.20606406627995694No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC335580.20517751939657583No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT329120.20122780017820205No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC327060.19996829219215712No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC323380.19771829734330024No Hit
GGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTG313380.19160418090618908No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG307110.18777062990012042No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT299430.18307498847641906No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC279730.17103017909531007No Hit
GACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTG276360.16896972185600365No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC272100.16636510825379427No Hit
GAGGAGCAGTGACATCACAGGAAAAACCACCAACCAAGGCCAAGGAGACC265350.16223807965874426No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT264710.16184677620676916No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACC259640.1587469191731538No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC258930.15831281690611892No Hit
GGTTCACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTG258360.15796431226920357No Hit
GTCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG253210.15481554230409134No Hit
GGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCATGGGCACCAGTC252010.154081848331638No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG250890.15339706729068156No Hit
GAGAGGACCCCAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGC247970.1516117452910451No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC245630.1501810420447611No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG241970.14794327542877841No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC240260.1468977615180324No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC235790.1441647514706437No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG234880.14360836687486658No Hit
GACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT231750.1416946484300508No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACCT227600.1391572901086497No Hit
GAGATACAGAAGACCCCTCCGTCCTGGAGCACCTGCCATGAGCATCAGCC225540.13789778212260478No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT222190.13584955311617256No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC216150.13215662678815743No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC213570.13057918474738273No Hit
GTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACT211150.12909956856960184No Hit
GAGGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGCATCCTG211080.12905676975454208No Hit
GAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGA209220.12791954409723938No Hit
GCTTTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAGAGG208140.1272592195220314No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC207750.12702076898098405No Hit
AGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATG207700.1269901983987985No Hit
GACAGTGACACTGATCTGATAAAGCCCCCATCCTGGCCTGACCCTGCCAT207020.12657443848107494No Hit
GATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCCT199490.12197050880393026No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT198450.1213346406944707No Hit
GGACTTCCTTGAGAGTCCTGTTCCCCTTTCATCAATGCACAGATACAGAA196140.11992227979749802No Hit
GATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTACCAATCTG195050.11925584110585291No Hit
GACAGTGACCCTGATCTGGCAAAGCTTCCATCCTGCCCTGACCCTGCCAT192830.11789850725681422No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC189690.11597867469556133No Hit
GCTGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGG189040.1155812571271491No Hit
ATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCTG188320.1151410407436771No Hit
GGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCT183690.11231020483329464No Hit
GGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGT182390.11151536969647019No Hit
GGAGGTCTCAGAATGACTTCCTTGAGAGTCCTGTTCCCCTTTCATCAATG179250.1095955371352173No Hit
GGATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC178010.10883738669701551No Hit
GGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTA177990.10882515846414129No Hit
GGCAGGTCCAGTGAATGCTGGTGTCACTCAGACCCCAAAATTCCGCATCC177960.10880681611482995No Hit
GGAGCCCCACCAGGGAGACCCAGTGACATAGATGGTCTGCTCAGGGTGAT176780.10808535037525084No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT174580.10674024475908639No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT171760.10501606392382104No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC166250.1016471857669728No Hit
GGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCCCTGTGTCTCC165010.10088903532877103No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)