FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5902598
Sequences flagged as poor quality0
Sequence length114-123
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT4711967.98285771790659No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT2990065.065667694123841No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT2607684.417851258039256No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT1685462.8554544964776527No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT1107281.8759197221291368No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC1006091.7044867361795601No Hit
GAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGC817611.385169716792504No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC795351.3474575093882388No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT767621.3004781962112277No Hit
GAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCT757451.2832484949847507No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT733931.2434016343311878No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC729341.235625397494459No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG600351.017094506520688No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT597951.013028500331549No Hit
AGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGT595541.008945552449955No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT512860.8688716392341135No Hit
GGCAGATGCAAATCAAGAGCCAACCATGCAAGCAGTCCCTTCTAAGGCCA456550.7734729690214377No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC420110.7117374417163426No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC392550.665046137311062No Hit
GGATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCAC364550.6176093984377726No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC351640.5957376734786953No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC347060.5879783783344216No Hit
GGAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGG328970.5573308566837857No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC323630.5482839929129512No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC287850.4876666173098693No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC282180.4780606776880282No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA277520.4701658490041165No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG274520.46508334126769263No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG258780.43841711734392214No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG240660.407718770615922No Hit
GCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTG238590.4042118402777895No Hit
GACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTC224030.37954473606367906No Hit
GAGAGCCTGAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCT217630.3687020528926415No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA212820.3605530988219086No Hit
GAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTG210220.35614825878367457No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG200870.34030777633848686No Hit
GGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC199850.3385797237081028No Hit
GATACACACAGTGACACTTCCATGAGCACAAGGGATGTCCTTCACTCTCT198780.3367669626154449No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG198110.33563186922097693No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG194550.3296006267070873No Hit
GATTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTC189690.32136696417408067No Hit
GCTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTT164630.2789110828824866No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT162960.2760818202425441No Hit
GACTATAGTGGGAACACTCCCAAAATTCAAGTTCGCAGATGCAAATCAAG161000.2727612485214138No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG160020.27110096266084865No Hit
CTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTT158800.2690340761813696No Hit
GACATACACACAGTGACACTTCCATGAGCACAAGGGATGTCCTTCACTCT144540.24487522274090154No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG141290.23936917269310903No Hit
ATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACTG139440.236234959588981No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC136130.23062725938646No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC134270.2274761045898772No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT128680.21800569850767407No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT128320.21739579757930322No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT125770.21307566600334296No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG124260.21051747044267627No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT122140.20692583164227005No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT120350.2038932686928705No Hit
GGACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCA119750.20287676714558575No Hit
GAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG119700.202792058683312No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT118190.20023386312264532No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT114400.1938129616822965No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC113610.19247456797837154No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC113020.19147500812354154No Hit
GAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGG112640.19083122381026119No Hit
GACAGACCATCAACCCACTGCCTGGTCCTGGGAGAAGACCTATTCTTTCT112540.1906618068857137No Hit
GGCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTG112250.19017049780452608No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG110750.18762924393631414No Hit
GGATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC109710.18586730792102055No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG108930.18454585590955033No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT107600.18229261081306908No Hit
GGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGT103680.17565146737080858No Hit
GAAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTC97500.16518150143377544No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC97390.16499514281677322No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC96600.16365674911284828No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG96580.16362286572793877No Hit
GTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTG93470.15835399937451272No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT93290.15804904891032728No Hit
GACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTG93150.15781186521596083No Hit
GTCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG90940.15406775118346192No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG90620.15352561702491005No Hit
GGAGCCTGAGACAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTG89290.1512723719284288No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA87870.14886665159985485No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC87630.14846005098094092No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG87620.1484431092884862No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCGTTTGT87180.14769767482047735No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC86720.14691835696755903No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA85950.14561384664854357No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG84310.1428354090859652No Hit
GAGAGTTATGGGAGGTGCGAATGACTCTGCTCTCTGTCCTGTCTCCTCAT81710.13843056904773118No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA80350.1361264988738857No Hit
GAGTCTAGACACACTAAGCCATGCTTACCAGTTACTATAGTGGGAACACT79370.13446621301332057No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC79140.1340765540868614No Hit
GGAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGG77310.13097622436764286No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC77170.1307390406732764No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT75250.12748623572196516No Hit
GGTCACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTG74970.12701186833323225No Hit
GCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACTGGA74920.1269271598709585No Hit
GAGACACACTAAGCCATGCTTACCAGTTACTATAGTGGGAACACTCCCAA74600.12638502571240665No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT74010.12538546585757662No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT73420.12438590600274659No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC71810.12165829351753245No Hit
GGCTCCTGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTG71360.12089591735706888No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG70860.12004883273433156No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC68410.11589811808291875No Hit
AAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCCT68230.11559316761873331No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA67380.11415312376007987No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCC67290.11400064852798716No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC66540.11273002159388121No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC65460.11090031880876862No Hit
GGTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCC64530.10932474141047722No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT63450.10749503862536464No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG61810.10471660106278625No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG61680.10449635906087457No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC61270.10380174967022995No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT61170.1036323327456825No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC61030.10339514905131604No Hit
GGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCT60840.10307325689467588No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA60830.10305631520222112No Hit
AGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGC60720.10286995658521893No Hit
AGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTG60360.10226005565684805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTCG251.3488352E-7116.8139651
GCGTGCA302.930392E-9116.8139651
TCGACCA251.3488352E-7116.8139651
GGATCTG69800.0107.609431
CACGTTA2500.0106.29961116-117
GAATGCG99150.0105.739321
GCTTCAA318950.0105.36881
GATACAC22550.0104.381431
GCTATTC28500.0103.698011
GACTATA20200.0102.356791
GTCTTTC103500.0100.4487151
GATTCCT584000.0100.251981
GCTTTTC52500.0100.237511
GAGAACT24600.0100.1940841
GACTGTG46000.0100.1806641
GGTCACT8800.099.557361
CGTTCAG1000.099.290192
GAGAGCT50150.097.946711
GATTCTT128600.097.7385941
GATACGT1800.097.344971
Produced by FastQC (version 0.11.5)