FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5902598
Sequences flagged as poor quality0
Sequence length151
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA3678736.232391228404848No Hit
TAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC2802054.747146934282158No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA2442024.137195180833931No Hit
TGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC1696302.873819291098598No Hit
AAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC1068591.8103723140217238No Hit
CGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG817141.384373457247131No Hit
CAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC752931.2755908499952056No Hit
CTTCTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGC607111.0285470906200964No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT415320.7036223710305191No Hit
CCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGAGCCCTG383720.6500866228735211No Hit
GTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGT380150.6440384386671767No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT350950.5945686966993179No Hit
GCCGAACACTTCTTTCTGCTTTCTTGACATCCGCTCACCAGGCCTGGGGG350070.5930778277633002No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA347850.5893167720383465No Hit
TGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA345780.585809841700214No Hit
TCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTG342820.5807951007336092No Hit
GTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCA320330.542693234402885No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG305150.5169757452565802No Hit
GGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC288220.4882934599306949No Hit
GCCAGGCAGGGTTGCTGGACACTCTGAGAGAAGAAAGGGTTAATCCCATG282990.47943295477686265No Hit
CGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC277970.4709282251645801No Hit
GGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCA262880.4453632112503681No Hit
CAGCGATTCTCATCTCAATGCCCCAAGAACGCACCCTGCAGCCTGGCAAT253370.4292516617259044No Hit
CAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCG214280.3630265859203015No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC204480.34642372731465026No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT202350.3428151468217893No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC184920.3132857768731667No Hit
AGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAG177940.30146047553975386No Hit
GGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT172790.2927355039255596No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA152650.2586149353217007No Hit
CAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGCTCCCT152070.25763231715932544No Hit
TGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA143490.2430963450331532No Hit
CATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGACCAGTGCCC142610.24160547609713554No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG137340.2326772041734843No Hit
CTCTAGAGAGAAGAAGGAGCGCTGCTCCCTGATTCTGGAGTCCGCCAGCA129240.21895443328513986No Hit
CTCTGCAGAGAGGACTGGGGAATCCGTCTCCACTCTGACGATCCAGCGCA125720.2129909575410692No Hit
TCACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC124800.21143232183523253No Hit
CTCTGCAGAGAGGCCTAAGGGAGCTTTCTCCACCTTGGAGATCCAGCGCA123140.20862000088774468No Hit
TAACTTCCACTCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC121920.20655311440826565No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG118530.20080988066610667No Hit
TAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGCAGGGGGACT117940.19981032081127667No Hit
ATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC115740.1960831484712325No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG114840.19455839615030535No Hit
CAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATCGG111290.18854409532887045No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT104740.17744728677101168No Hit
CGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCA99910.16926444931536927No Hit
TCGATTTTCTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCC99540.16863760669454367No Hit
AAGCGATTCTCATCTCAATGCCCCAAGAACGCACCCTGCAGCCTGGCAAT95860.16240306387119705No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC95710.16214893848437587No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA91860.15562638688929858No Hit
CGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCTTGG87240.14779932497520584No Hit
CTCTCGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAGTCCGCCAGCA85850.14544442972399613No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC85420.144715936948442No Hit
CTTCTTTGCAGAAAGGCCTGAGGGATCCGTCTCCACTCTGAAGATCCAGC85380.14464817017862303No Hit
TGAGGTGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC84840.14373331878606674No Hit
TCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC84040.14237798338968705No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC81450.13799008504390778No Hit
TGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAA80140.13577072333233603No Hit
CTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCC78410.13283981053766494No Hit
CAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGG77500.13129811652428305No Hit
CAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTCTG75070.12718128525777972No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC74520.12624949217276865No Hit
CAACTTCCACTCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC72770.12328469599318809No Hit
CCACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACATCC71560.1212347512061638No Hit
TCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTC67860.1149663249979077No Hit
TGAGTGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC67670.11464443284126752No Hit
TGCTGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGT66360.11242507112969578No Hit
CGAGGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTC66000.11181517020132492No Hit
CTTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG64750.10969745864448162No Hit
CCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGGAGCTGGGGGACT64050.10851154017264941No Hit
GGATCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGA62950.10664795400262732No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA62640.10612276153653018No Hit
TGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCACAG60600.10266665627576196No Hit
AGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAGAGC60020.10168403811338668No Hit
CGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTCTGG59510.10082001179819461No Hit
CTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGGAGT59460.1007353033359209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTA331300.0144.25989145
GGTCACA622150.0144.07312145
TTCCAAT723600.0144.057595
CGTACTG425300.0144.0445145
TCCAATC741400.0143.902736
CTTCCAA729150.0143.894444
CGTTGTC390800.0143.86899145
CGTGCTC271900.0143.80887145
GGTGCTC1055250.0143.68361145
AATCCAG745650.0142.258549
AAACTTC158400.0142.240891
CAATCCA742850.0142.218968
AGTTGTC154450.0141.88425145
CCAATCC753800.0141.768757
CGTTGTA279650.0141.50839145
TAACTTC434500.0141.31631
CGCTAAG133800.0141.031681
AGTGCTC562000.0139.48708145
CGTGCTG106050.0139.10338145
GGTCACC757950.0137.20634145
Produced by FastQC (version 0.11.5)