FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7955761
Sequences flagged as poor quality0
Sequence length114-124
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG1559701.9604661326553172No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1486071.867916846672493No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1200961.5095476095875682No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT1100281.3829978049868517No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC1045201.313764955985983No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC976051.2268468095006877No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT839151.0547702476230747No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG784200.9857008022236968No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC666310.8375188746871607No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC616980.7755134926753078No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCAT574550.7221810710502741No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT570190.716700765646429No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT535970.6736879099309293No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT517660.6506731411363413No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC515490.6479455579422257No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG492980.6196515958687044No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG490270.6162452592530118No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT479650.6028964419619945No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA477340.5999928856585812No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG464360.5836776645251158No Hit
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT446430.5611405370271932No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG439900.5529326484292326No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC431550.5424371094104008No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG418170.525619107964656No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG408220.5131124476967068No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG398370.5007314825068274No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC394440.4957916659386827No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG359190.4514841509190635No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG357320.44913365295915747No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC331150.41623925102828No Hit
GGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGAC330680.4156484841613518No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT318460.4002885456212172No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT315180.3961657470605263No Hit
GAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTTTCC312040.3922189216091333No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC304070.382201023886967No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC295340.37122784357146976No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT272040.34194089038119674No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC270020.3394018498041859No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGTCTGACACTGTCAT263240.33087972351105066No Hit
GACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGC261790.329057144879038No Hit
GGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGACACCAGA253180.3182347986572246No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC251290.31585916168170464No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT248980.3129556053782913No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC239390.3009014473913935No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG237490.29851324090806647No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT228570.2873012399442366No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG220850.2775975799172449No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG219400.2757750012852322No Hit
GAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCAT214520.2696410814754239No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG210740.2648898075243839No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA207660.26101839911983277No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT204960.2576246320119471No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA204140.25659393237177436No Hit
GATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGC201650.2534641249278353No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG195020.24513054125180483No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG193050.24265434821382895No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA191500.2407060745037464No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTA190450.23938627618401306No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT189820.23859439719217307No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT188800.2373123073958607No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC188080.23640730283375783No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC178430.22427772780001812No Hit
GAGATCTCCCAGAGGGAGCAGCCTGACCACATCACTGGCCCAGAAGAGGA177500.22310876357396858No Hit
GATCCAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCC176090.22133646297318385No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC175700.22084625216871145No Hit
CAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG174370.21917450763038257No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC170050.2137444802577654No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG168460.21174592851645493No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC165910.20854070402567398No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT165020.20742201783085237No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG163580.20561200870664667No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC161460.202947273051566No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC157150.19752981518675589No Hit
GGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGG154610.1943371602037819No Hit
GAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC150770.18951046920589998No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC150210.18880657676870885No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT143820.180774661280046No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG141520.17788367448443965No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT139350.17515609129032408No Hit
GAGTCCTCTCACCACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGT138970.1746784499936587No Hit
TTCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTC138170.17367288936909997No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT130580.16413263294359898No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA129730.16306422478000535No Hit
AGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGC128560.16159359236658818No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC127520.16028636355466183No Hit
GATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCCAGAG125120.15726968168098565No Hit
GAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC123330.15501973978353548No Hit
GGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTC122600.15410216571362562No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG121080.15219160052696407No Hit
GATCCTGCCCTGACTCTGTCATGGGCACCAGGCTCCTCTGCTGGGCAGCC120330.15124888744144024No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC118480.14892352849714818No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT117470.14765400820864277No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC116770.14677414266215388No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC114940.14447392273347578No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA114430.1438328778353196No Hit
GAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCT113750.14297815130444466No Hit
GCTTCCCCAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCC113560.14273933065611197No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG112790.14177147855497418No Hit
GCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCC110030.13830229440024655No Hit
GAGAACTCACTGGGTTCTTCCCCAGGAGGACCAAGCCCTGAATCAGGTGC109800.13801319572068593No Hit
GAGAATGACTTCCTTGAGAGTCCTGTTCCCCTTTCATCAATGCACAGATA109510.13764867999428337No Hit
GCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTG104800.13172844181719387No Hit
GATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATG104410.13123823101272147No Hit
GAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCA104300.13109996642684466No Hit
AGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTCT102520.12886259403720146No Hit
GGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAG101090.12706515442080274No Hit
GGAGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCT100780.12667549967878622No Hit
GGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTACCATGGGCACCAGTC99730.12535570135905288No Hit
ACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG99090.1245512528594059No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC98300.12355826174265416No Hit
GACAGACCCAGAAGACCCCTCCATCCTGTAGCACCTGCCATGAGCATCGG97980.12315603749283068No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT97700.1228040912742351No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC95860.12049130183775002No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG95840.12046616282213607No Hit
GCTATTTCCCCAGGCAGGGCTGGGAGAGATGAGATCCTGGCCTGGACCTG95000.11941032416634939No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC94400.11865615369793035No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT94180.11837962452617669No Hit
GAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCG93560.11760031504214367No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC93270.11723579931574113No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA92210.11590343148820083No Hit
GGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTC89740.11279876305987573No Hit
GGCTCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCA88430.1111521575371608No Hit
ATTCTGTGATCAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCAC87250.10966895561593668No Hit
GAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTG86100.10822346221813349No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT85210.10710477602331192No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA84340.1060112288441043No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG83150.10451545741507318No Hit
GGTTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAG82790.10406295513402175No Hit
GCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCG82430.10361045285297033No Hit
AGAAGACCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGT81760.10276829582990238No Hit
GAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTG81400.10231579354885095No Hit
GCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCCA81180.10203926437709729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCG15600.0103.1162641
GCTTCAA63250.0102.0099951
GTCTTTC47450.0101.703721
GAGAGCT134750.0100.093571
GGATCTG96300.099.429721
GACTGCA10150.098.6897351
GCCCGAC10100.098.59491
GAGAACT35450.098.566111
GAAGCTA6100.098.5279541
GCTATTT13700.098.062611
GACAACT35300.097.9834061
GGACGTG19950.097.762931
AGTGGTA569900.097.04332
GCTATTC13650.095.831761
AGTCGTA1850.095.550552
GAGTGAT57150.094.855111
GACTTCG1000.094.277731
GCTTTTC59850.093.037231
GAGAGAC19750.092.4876561
AGTCATT13600.091.850884
Produced by FastQC (version 0.11.5)