FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912462
Sequences flagged as poor quality0
Sequence length112-122
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC1364253.486934825181689No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT959462.452317747750649No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGT590281.5087175287581067No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT409681.047115601378365No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT352140.9000470803294703No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG300880.769029833388797No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG267220.6829970489170246No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC263470.6734122912887077No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC256680.6560574901430353No Hit
ATATCTGTCAACGTGGAACCTCCCTGACGATCCAGTGTCAAGTCGATAGC251090.6417698114384242No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC248130.6342042427504727No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG247660.6330029531277237No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC235040.6007470487892279No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT219110.5600309983841377No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG218850.5593664551885744No Hit
GCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTT209450.5353406627335933No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT204260.5220753581760027No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC198860.5082733071912264No Hit
GACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGT187900.48026025556286556No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG179200.4580236178651703No Hit
GAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACG170040.43461124989840155No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC168960.43185083970144633No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC164630.4207836395599497No Hit
GAATGCGATCATGGGCACCAGGCTCCTCGGCTGTGCAGCCCTGTGTCTCC161510.4128091212131901No Hit
GCTTTTCACCAATGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCT161030.4115822722367655No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA159080.4065981982700407No Hit
GCTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGC155850.3983425270328504No Hit
GCTCTCACCACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGC152560.389933499673607No Hit
GCTTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC150250.38402928897456384No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG134870.3447190030216268No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC127470.3258050813017481No Hit
GCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCT123260.3150445934043577No Hit
GAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG119850.30632885380100816No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA119120.3044630209826958No Hit
GTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCT111000.2837088257981803No Hit
CAGAAAGTCGAGTTTCTGGTTTCCTTTTATAATAATGAAATCTCAGAGAA110650.28281424841953734No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC110490.2824052987607292No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGCC109810.2806672627107944No Hit
ATGATGTTCTGGTACCGTCAGCAACCTGGACAGAGCCTGACACTGATCGC107940.2758876635734737No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT107830.27560651068304304No Hit
CATGCTGATGGCAACTTCCAATGAGGGCTCCAAGGCCACATACGAGCAAG104070.26599619370105065No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT102830.2628268338452872No Hit
GCTATTTCCCCAGGCAGGGCTGGGAGAGATGAGATCCTGGCCTGGACCTG102390.26170222228356466No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG100790.25761272569548277No Hit
GGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGA100340.2564625547800848No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT96170.2458043042973964No Hit
GCCCCGACGTGCTGCAGCAAGTGCCTTTGCCCTGCCTGTGGGCTCCCTCC88630.22653255162606054No Hit
GACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTCGG88170.22535682135698698No Hit
GCTTCCCCAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCC86940.22221302085489902No Hit
AGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAG81950.20945890337081868No Hit
GGCCTTTGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGAC80690.2062384248077042No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCCCCT80660.20616174674667767No Hit
CAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAG80390.20547164419743882No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG80360.2053949661364123No Hit
GCCTTTTCACCAATGCACAGACCCAGAGGACCCCTCCATCCTGCAGTTCC78460.2005386889380651No Hit
GCACTACGATTTCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTG76750.19616803945955258No Hit
CTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCC76640.19588688656912195No Hit
GCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTC76030.19432776599491575No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCT71670.1831838877923926No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT71450.18262158201153136No Hit
GCACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCT69440.1774841519227535No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG68850.1759761500558983No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT68520.17513269138460644No Hit
GCATCCTGCCCTGACCCTGCCATGGGCACCAGGCTCCTCTTCTGGGTGGC67940.17365024887142674No Hit
GCTCATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGC65610.1676949194650325No Hit
GAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGCCCCACTGTGC62990.16099836880204843No Hit
GAGAAGGGAAAGGATGTAGAGCTCAGGTGTGATCCAATTTCAGGTCATAC62830.16058941914324024No Hit
AGTGTCAAGTCGATAGCCAAGTCACCATGATGTTCTGGTACCGTCAGCAA60230.15394398718760718No Hit
GACTACTGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGG60140.1537139530045276No Hit
GCTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTT59190.15128581440535396No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA56810.1452026882305822No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC55760.14251895609465343No Hit
GCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTG54550.13942627429991652No Hit
GCTTTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGC54130.13835278144554503No Hit
ATGAAAATATGTTCTGGTATCGACAAGACCCAGGTCTGGGGCTACGGCTG53180.1359246428463714No Hit
GCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACCT51350.13124728112375278No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG50460.1289724986466322No Hit
AGTCGATAGCCAAGTCACCATGATGTTCTGGTACCGTCAGCAACCTGGAC49500.1265188006937831No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT49380.12621208844967696No Hit
GCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTACCTG49050.12536862977838506No Hit
ATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCTG48870.12490856141222585No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT48210.12322164406964208No Hit
TCCAATTTCAGGTCATACTGCCCTTTACTGGTACCGACAGAGCCTGGGGC47670.12184143897116444No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCCCC47580.12161140478808485No Hit
GCTCCATCCTGCAGTTCCTGCCATGAGCCTCGGGCTCCTGTGCTGTGGGG45810.11708739918751927No Hit
AGTCACCATGATGTTCTGGTACCGTCAGCAACCTGGACAGAGCCTGACAC45540.11639729663828044No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA45460.11619282180887636No Hit
GCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGG45230.11560495667433958No Hit
GGCTCATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCA45020.11506821024715383No Hit
GTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC44940.11486373541774976No Hit
GACTGGAAATGGTGACCTCACAGGAAGATGCATCTTGTAGGAGGCAGCTG44840.11460814188099464No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT44680.11419919222218644No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC44390.11345797096559661No Hit
GCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCGTTTGT44050.11258895294062919No Hit
GGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGG44030.11253783423327818No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC43570.11136210396420464No Hit
AGGATATGGACCATGAAAATATGTTCTGGTATCGACAAGACCCAGGTCTG43370.11085091689069439No Hit
GGTCCTGTCTCCTCATCTGCAAAATTAGGAAGCCTGTCTTGATTATCTCC43000.10990522080470047No Hit
TGATGTTCTGGTACCGTCAGCAACCTGGACAGAGCCTGACACTGATCGCA42860.10954738985324329No Hit
CTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGTGTGGCCTTTTGTT42810.10941959308486575No Hit
GGCCCTGCCTGTGGGCTCCCTCCATGGCCAACTCTGCTATGGACACCAGA42710.10916399954811064No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC42340.10821830346211668No Hit
GTCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCC42090.1075793196202289No Hit
GCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCCA42040.10745152285185135No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATC40330.10308087337333884No Hit
GCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCA40100.10249300823880207No Hit
GCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCGTCCTGGAGCACC39820.10177734633588774No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT39700.10147063409178159No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG39640.10131727796972852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)