FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005298898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3841709
Sequences flagged as poor quality0
Sequence length151
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACC811382.1120287871882018No Hit
CGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACC707731.8422269880409994No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCAACATGA506491.3183976193928275No Hit
GTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGC328400.8548278903998195No Hit
CGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG298510.7770239755275582No Hit
CCCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG247070.6431252341080493No Hit
CTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA227100.591143160504869No Hit
CTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCT222380.578856961836516No Hit
CTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGT208330.542284696732626No Hit
GCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGG187950.48923538977054226No Hit
TGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG137760.35859040859159297No Hit
GGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACC133620.34781395467485954No Hit
CTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCA131890.34331075050192505No Hit
GGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACAG131090.3412283439479669No Hit
CCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCC126610.32956686724580125No Hit
CTCAGTTGAAAGGCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCA114430.29786222746178853No Hit
CTTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGC107620.2801357416712198No Hit
CGGGTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGG106880.27820951560880847No Hit
CGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTCAACC105460.2745132439755328No Hit
GTACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGT101040.263007947764914No Hit
CGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG100490.2615762932590678No Hit
CTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGT99230.2582965029365837No Hit
CTCTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCA80560.20969833998358542No Hit
CCGGTTCTCTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCC79710.20748578302000492No Hit
CTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCC79550.20706930170921325No Hit
CTTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCT79490.2069131212176664No Hit
CTTCTCTGCAGAGAGGACTGGGGGATCCGTCTCCACTCTGACGATCCAGC78990.20561161712144257No Hit
CTTCTCAGGTCACCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAACG76270.19853143483798485No Hit
CAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAGTGA68330.17786354978995025No Hit
GAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCT67020.1744536090578438No Hit
CATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTCTGAGCAACATGA66630.1734384358627892No Hit
CTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAGCACCT66460.1729959244700731No Hit
CCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGG64500.16789402841287562No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACA63080.1641977567795999No Hit
CTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCCC57310.14917839950917677No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC55320.14399841320620588No Hit
CGAGGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGA55190.14366002214118767No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA54040.14066656271987285No Hit
GCGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGA53490.13923490821402662No Hit
TGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTCAACC51510.1340809519929802No Hit
TGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG50840.13233693650404027No Hit
CTTCTCACCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACG50620.13176427470170177No Hit
GGATCGATTCTCAGCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGA49490.12882287544423587No Hit
CAAGTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGA47830.12450188184477272No Hit
GGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACAG45910.11950410611527318No Hit
CGAACGATTCTCCGCACAACAGTTCCCTGACTTGCACTCTGACCTAAACC44780.1165627068578073No Hit
CTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCAGCGCA43740.11385557833766172No Hit
CAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGC38720.10078847721157431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCTC453950.0143.66939145
AGTTGTC210900.0142.67592145
CGTGCCC165150.0142.21355145
CGAACGA215450.0141.558011
CGTGCTA66750.0141.17787145
GAACGAT407200.0137.86882
CGTTGTC119150.0136.33969145
GGTGCTC148850.0136.21288145
AGTGCTC414150.0136.15721145
CGTACTG477700.0136.02576145
AACGATT393050.0135.7683
TGAACGA163900.0135.303481
GGAACGA50150.0134.871191
CCGGTTC26400.0133.456831
GGTGCCC47750.0132.83398145
GGTCACA46400.0132.01196145
ACCGATT17950.0131.662353
CGTGCGC40250.0131.65155145
AGTTGCC89050.0131.4659145
ACGATTC428400.0131.130784
Produced by FastQC (version 0.11.5)