FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005298899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005298899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3941249
Sequences flagged as poor quality0
Sequence length108-122
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCAT1777714.510524455572332No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT1265533.2109871769076253No Hit
GATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTCT1203603.053854247727053No Hit
GACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCAT875722.221935229162126No Hit
GAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTG647891.6438697478895652No Hit
GAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTG612121.5531117166157227No Hit
GCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC535791.3594421463855748No Hit
GACTTCTTAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTG512461.3002477133517827No Hit
GACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAG423921.0755981162316819No Hit
GACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCT412591.0468508840725363No Hit
GAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCC398281.0105425970295203No Hit
GAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTGC374110.9492168599345031No Hit
GATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACTCT304160.7717350515027089No Hit
GGCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTG283390.7190360213221747No Hit
GAGTGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTC266180.6753696607344525No Hit
GAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCT254830.6465716832405158No Hit
GACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC236990.6013068446068746No Hit
GAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCTG233300.5919443303379208No Hit
GAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTG217310.5513734351724542No Hit
GAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGG208550.5291469785339622No Hit
GACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACTCTGTCAT206060.522829184352473No Hit
GATCACTTGAAGATGCTGAGTCTTCTGCTCCTTCTCCTGGGACTAGGCTC199740.5067936585584925No Hit
GAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCTGC189150.4799240037866169No Hit
GGCTCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCA179700.4559468330978327No Hit
GACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATCGG175160.44442764210025804No Hit
GCTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT171370.4348114011573489No Hit
GATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCAAT164930.41847140335462185No Hit
GGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCT155030.3933524626330384No Hit
GGCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC152050.38579140774916787No Hit
GAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGT152010.3856899170795857No Hit
GAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGTTCTCT144190.3658484911762743No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGCC144000.3653664104957591No Hit
AGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATG143800.36485895714784833No Hit
AGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACA140390.3562068775659696No Hit
GACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCAT136520.3463876552838961No Hit
GGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTCC134880.3422265378310277No Hit
GAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTG128170.32520147800862115No Hit
GGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCATGGGCACCAGTC127620.3238059813018665No Hit
GACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCT126670.3213955778992903No Hit
GAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCT123350.3129718523239714No Hit
GGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC113510.2880051476067612No Hit
GAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTCTG113030.2867872595717753No Hit
GAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGC112300.284935054851901No Hit
GAGGAGAACCAAGCCCTGAATCAGATGCAGTGCTTCCTGTCCCTCTGTGC111790.2836410488147285No Hit
GGCTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTG111190.28211868877099616No Hit
GGCTTTCTCACCCCAGGAGACCAGCAACCTGAGCAGGGAGATGCTTAGTC97610.24766260644785448No Hit
GGAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCC95420.24210599228823146No Hit
GGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCA95170.241471675603343No Hit
GACAGACCCAGAAGACCCCTCCGTCCTGCAGCCCCTGCCATGAGCCTCGG92470.23462105540654749No Hit
ACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGG90780.23033307461670147No Hit
GAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG88980.2257659944855045No Hit
GAGCTCATGGGGTAGGATTCCTCTGCTCTGGCAGCAGATCTCCCAGAGGG88390.22426900710916767No Hit
GGCTGTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG86580.21967655431057517No Hit
GAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGTGGCCTTTTGTCTC85960.21810344893205175No Hit
GATCCAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCC84300.2138915861443923No Hit
GCATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC82940.21044090337859903No Hit
GAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCCTGCCATGG82210.20858869865872467No Hit
GACAGGACCAGATGCCTGAGCTAGGAAAGGCCTCATTCCTGCTGTGATCC81700.20729469262155223No Hit
GACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTGACCCTGCCAT81060.20567084190823773No Hit
GTCACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC80110.20326043850566156No Hit
GGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCTGCC78790.1999112464094504No Hit
GATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCC78280.19861724037227793No Hit
GATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATGGCC72490.18392646595026094No Hit
GGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCAC71970.18260708724569294No Hit
ACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATG69560.17649227440336807No Hit
GAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACA68350.17342218164850787No Hit
GCTTTCCCAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCC68320.17334606364632124No Hit
GGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTT67230.17058044290020752No Hit
GCTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGC66780.1694386728674083No Hit
GGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCT65200.16542979141891315No Hit
GCTTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC65070.16509994674277112No Hit
GAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCATGG64900.16466861139704697No Hit
GGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCT64640.16400892204476297No Hit
GGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCCTG63730.16170000931176892No Hit
GTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCC61800.15680308450442995No Hit
GTTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTC61090.15500162511934668No Hit
GGCTCCTGCTCACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTG61070.1549508797845556No Hit
GGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCACCAGGC60330.15307330239728573No Hit
AGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTCTGCTGTGT58940.14954650162930583No Hit
GATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGCGGT58860.14934352029014153No Hit
AGACGTTACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCT58670.14886143960962628No Hit
GAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCCCC58110.14744057023547613No Hit
AGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGG57170.14505553950029546No Hit
GGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGGGCA56670.1437869061305185No Hit
ACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCCATG56510.1433809434521899No Hit
GAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCCATGG56200.14259439076292818No Hit
GACTCTGCCATGGGCTGCAGGCTCCTCTGCTGTGTGGTCTTCTGCCTCCT55420.14061532270607618No Hit
GCCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCT54710.1388138633209929No Hit
GGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCC51940.13178563445242866No Hit
GAGAGTGGGAAGGAACTACGCTGTAGGAAGGAAAGATGAACTTGAGTTTC51080.1296035850564123No Hit
GGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCAGGC50980.12934985838245694No Hit
GACTGTGGGAACTGCTCTGTGGCGACAAGGACGTCCCTCATCCTCTGCTC50470.12805585234528444No Hit
GAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC50430.1279543616757023No Hit
ACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTCT49640.12594992095145474No Hit
GACCTGTTAGAGGAGGGAAACAGAGTGGGAAGGAACTACGCTGTAGGAAG48400.12280371019440792No Hit
GAGAATGTCTCAGAATGACTTCCTTGAGAGTCCTGCTCCCCTTTCATCAA48310.12257535618784805No Hit
GATTCCTGTATGGGGTGGTATTCCTGCCATGGGTCCTGGGCTTCTCCACT48180.12224551151170605No Hit
GGAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCA46840.11884557408070384No Hit
GACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTCTCT45730.11602920799979904No Hit
GGATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACTC45160.11458296595825335No Hit
AGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGTGGT45100.11443072995388011No Hit
GACTCTGCCATGGGCTCCTGGACCCTCTGCTGTGTGTCCCTTTGCATCCT44910.11394864927336487No Hit
GGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCT44320.11245166189702807No Hit
GTCACAGTGACACTGATCTGGTAAAGCCCTCATCCTGTCCTGACCCTGCC42790.10856964378551062No Hit
GACTCTGAGAGGGGCTGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAG42150.10694579307219615No Hit
GAGAGTCCTGCTCCCCTTTCATCAATGCACAGATACAGAAGACCCCTCCG42130.10689504773740507No Hit
GAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTCTTC41460.10519507902190398No Hit
GTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGCTCCTTC41220.10458613500441104No Hit
GACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCAT41140.10438315366524673No Hit
GAGACACCAGCCCCAAGCTAGGAGATCCTGCCATGGGCTTCAGGCTCCTC39810.10100858890164006No Hit
GATCCTGTCCTGACCCTGCCATGGGCACCAGTCTCCTATGCTGGGTGGTC39760.10088172556466236No Hit
GAGGTGCAGTGCTGCCTGCCCCACTGTGCCATGGGCCCTGGGCTCCTCTG39480.10017129087758728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)